Information on EC 1.1.1.40 - malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

for references in articles please use BRENDA:EC1.1.1.40
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
1.1.1.40
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RECOMMENDED NAME
GeneOntology No.
malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-malate + NADP+ = pyruvate + CO2 + NADPH
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
C4 and CAM-carbon fixation
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gluconeogenesis
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photosynthesis
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Pyruvate metabolism
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Carbon fixation in photosynthetic organisms
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Metabolic pathways
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Microbial metabolism in diverse environments
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C4 photosynthetic carbon assimilation cycle, PEPCK type
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gluconeogenesis I
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syringate degradation
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methylgallate degradation
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protocatechuate degradation I (meta-cleavage pathway)
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C4 photosynthetic carbon assimilation cycle, NADP-ME type
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SYSTEMATIC NAME
IUBMB Comments
(S)-malate:NADP+ oxidoreductase (oxaloacetate-decarboxylating)
The enzyme catalyses the oxidative decarboxylation of (S)-malate in the presence of NADP+ and divalent metal ions, and the decarboxylation of oxaloacetate. cf. EC 1.1.1.38, malate dehydrogenase (oxaloacetate-decarboxylating), and EC 1.1.1.39, malate dehydrogenase (decarboxylating).
CAS REGISTRY NUMBER
COMMENTARY hide
9028-47-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain NRRL 2270
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Manually annotated by BRENDA team
strain NRRL 2270
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Bothriochloa biloba
strain Kuntze
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Manually annotated by BRENDA team
Bothriochloa biloba Kuntze
strain Kuntze
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Manually annotated by BRENDA team
strain Kuntze
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Manually annotated by BRENDA team
strain Kuntze
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Manually annotated by BRENDA team
cultivars Vergasa and Biela
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
; from the Baltic Sea
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Cymbopogon ambiguus
Spreng.
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Manually annotated by BRENDA team
Cymbopogon ambiguus Spreng.
Spreng.
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Manually annotated by BRENDA team
Cymbopogon bombycinus
Spreng.
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Manually annotated by BRENDA team
Cymbopogon bombycinus Spreng.
Spreng.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Digitaria smutsi
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene maeB
UniProt
Manually annotated by BRENDA team
isozyme ChlME1, nuclear encoded, 3'-untranslated region; C3-C4 species, 2 isozymes ChlME1 and ChlME2; isozyme ChlME2, nuclear encoded, 3'-untranslated region; C3-C4 species, 2 isozymes ChlME1 and ChlME2
SwissProt
Manually annotated by BRENDA team
cultivar Mara des Bois
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
constitutive CAM plant, 2 isozymes
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Manually annotated by BRENDA team
Mnium undulatum
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
wild-type plants and transgenic plants, expressing potyviral helper component protease HC-pro or Potato virus Y strain NTN
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Pigeon
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
five NADP-ME genes, PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
salmon
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Manually annotated by BRENDA team
seagull
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Manually annotated by BRENDA team
gene Sco5261
UniProt
Manually annotated by BRENDA team
gene Sco5261
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain TV 7-37
SwissProt
Manually annotated by BRENDA team
strain TV 7-37
SwissProt
Manually annotated by BRENDA team
hexaploid wheat
UniProt
Manually annotated by BRENDA team
; grown in male Wistar rats
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Manually annotated by BRENDA team
grown in male Wistar rats
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-malate + NAD(P)+
pyruvate + CO2 + NAD(P)H
show the reaction diagram
(S)-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
(S)-malate + NADP+
?
show the reaction diagram
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-
?
(S)-malate + NADP+
pyruvate + CO2 + NADPH
show the reaction diagram
(S)-malate + NADP+
pyruvate + CO2 + NADPH + H+
show the reaction diagram
(S)-malate + NADP+
pyruvate + NADPH + CO2
show the reaction diagram
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-
?
(S)-malate + NADP+
pyruvate + NADPH + H+ + CO2
show the reaction diagram
Oxaloacetate
Pyruvate + CO2
show the reaction diagram
oxaloacetate + NADP+
pyruvate + CO2 + NADPH
show the reaction diagram
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?
pyruvate + CO2 + NADPH
L-malate + NADP+
show the reaction diagram
pyruvate + NADPH
?
show the reaction diagram
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at 5.4% of the activity of NADP-dependent oxidative decarboxylation of malate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-malate + NAD(P)+
pyruvate + CO2 + NAD(P)H
show the reaction diagram
P16243
the unique and specialized C4-type enzyme has evolved fro the C3-type enzyme
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?
(S)-malate + NAD+
pyruvate + CO2 + NADH
show the reaction diagram
Q5JGC7
when the R221G/K228R/I310V mutant is used with NADH, the mutant gives 1.2 and 2.7 times higher malate concentration than the wild-type with NADPH and NADH, respectively. These results can be partly explained by the alteration of the cofactor preference of the mutant enzyme, since the half-life of NADH is approximately 1.3times longer than that of NADPH at 50°C. However, the Km of the triple mutant for NAD+ remains 190times higher than that of the wild-type for NADP+
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r
(S)-malate + NADP+
pyruvate + CO2 + NADPH
show the reaction diagram
(S)-malate + NADP+
pyruvate + CO2 + NADPH + H+
show the reaction diagram
(S)-malate + NADP+
pyruvate + NADPH + H+ + CO2
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
Pigeon
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weak activation
Fe2+
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activates
KCl
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activates at 2 mM
NH4+
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activates
NH4Cl
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activates
additional information