BRENDA - Enzyme Database show
show all sequences of 1.1.1.397

In vitro reconstitution of indolmycin biosynthesis reveals the molecular basis of oxazolinone assembly

Du, Y.; Alkhalaf, L.; Ryan, K.; Proc. Natl. Acad. Sci. USA 112, 2717-2722 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BW25113 cells
Streptomyces griseus subsp. griseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus ATCC 12648
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces griseus subsp. griseus
A0A0D4BSN8
-
-
Streptomyces griseus subsp. griseus ATCC 12648
A0A0D4BSN8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus ATCC 12648
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BW25113 cells
Streptomyces griseus subsp. griseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
Streptomyces griseus subsp. griseus ATCC 12648
-
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
(2S,3R)-2-hydroxy-3-(indol-3-yl)butanoate + NAD+
-
739549
Streptomyces griseus subsp. griseus ATCC 12648
(R)-3-(indol-3-yl)-2-oxobutanoate + NADH + H+
-
-
-
?
Other publictions for EC 1.1.1.397
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739549
Du
In vitro reconstitution of ind ...
Streptomyces griseus subsp. griseus, Streptomyces griseus subsp. griseus ATCC 12648
Proc. Natl. Acad. Sci. USA
112
2717-2722
2015
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-