Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a His-tagged protein | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxaloacetate | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii | |
0.02 | - |
oxaloacetate | native enzyme, at pH 7.4 and 30°C | Rasamsonia emersonii | |
0.022 | - |
oxaloacetate | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
0.022 | - |
oxaloacetate | recombinant enzyme, at pH 7.4 and 30°C | Rasamsonia emersonii | |
0.025 | - |
NADH | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii | |
0.025 | - |
NADH | native enzyme, at pH 7.4 and 30°C | Rasamsonia emersonii | |
0.029 | - |
NADH | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
0.029 | - |
NADH | recombinant enzyme, at pH 7.4 and 30°C | Rasamsonia emersonii | |
0.12 | - |
NAD+ | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii | |
0.12 | - |
NAD+ | native enzyme, at pH 7.4 and 30°C | Rasamsonia emersonii | |
0.13 | - |
NAD+ | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
0.13 | - |
NAD+ | recombinant enzyme, at pH 7.4 and 30°C | Rasamsonia emersonii | |
1 | - |
L-malate | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
1.25 | - |
L-malate | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rasamsonia emersonii | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Rasamsonia emersonii |
70000 | - |
gel filtration | Rasamsonia emersonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | Q8TG27 | mitochondrial malate dehydrogenase cDNA sequence | - |
Rasamsonia emersonii | Q8X1C8 | m-MDH chromosomal DNA sequence | - |
Purification (Comment) | Organism |
---|---|
- |
Rasamsonia emersonii |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1610 | - |
- |
Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Rasamsonia emersonii | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | - |
Rasamsonia emersonii | L-malate + NAD+ | - |
? | |
oxaloacetate + NADH + H+ | - |
Rasamsonia emersonii | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Rasamsonia emersonii |
Synonyms | Comment | Organism |
---|---|---|
m-MDH | - |
Rasamsonia emersonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 56 | reduction of oxaloacetate, recombinant enzyme | Rasamsonia emersonii |
52 | - |
reduction of oxaloacetate, native enzyme | Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
complete inactivation of native enzyme after 1 h, complete inactivation of recombinant enzyme after 15 min, half-life is 30 min in absence of bovine serum albumin, less than 10% loss of activity after 30 min in presence of 0.2 mg/ml bovine serum albumin | Rasamsonia emersonii |
60 | - |
10 min, complete inactivation | Rasamsonia emersonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2770 | - |
NAD+ | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii | |
2900 | - |
L-malate | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii | |
3100 | - |
NAD+ | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
3500 | - |
L-malate | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
81000 | - |
oxaloacetate | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
90000 | - |
NADH | pH 7.4, 30°C, recombinant enzyme | Rasamsonia emersonii | |
91000 | - |
oxaloacetate | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii | |
110000 | - |
NADH | pH 7.4, 30°C, native enzyme | Rasamsonia emersonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reduction of oxaloacetate, native enzyme and recombinant enzyme | Rasamsonia emersonii |
10 | - |
oxidation of malate, native enzyme and recombinant enzyme | Rasamsonia emersonii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rasamsonia emersonii |