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Literature summary for 1.1.1.244 extracted from

  • Lee, J.Y.; Park, S.H.; Oh, S.H.; Lee, J.J.; Kwon, K.K.; Kim, S.J.; Choi, M.; Rha, E.; Lee, H.; Lee, D.H.; Sung, B.H.; Yeom, S.J.; Lee, S.G.
    Discovery and biochemical characterization of a methanol dehydrogenase from Lysinibacillus xylanilyticus (2020), Front. Bioeng. Biotechnol., 8, 67 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.244

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The expressed enzyme contains a 6-histidine tag at the N-terminus Lysinibacillus xylanilyticus

Protein Variants

Protein Variants Comment Organism
A164F mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type Lysinibacillus xylanilyticus
S101V mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type Lysinibacillus xylanilyticus
T141S mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type Lysinibacillus xylanilyticus

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Lysinibacillus xylanilyticus
Cu2+
-
 Lysinibacillus xylanilyticus
Zn2+
-
 Lysinibacillus xylanilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 NAD+ pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.25
-
 ethanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.5
-
 butanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
1.09
-
 n-Propanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
1.19
-
 ethanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
1.38
-
 ethanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
1.82
-
 n-Propanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
2.33
-
 n-Propanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
3.23
-
 methanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
9.45
-
 n-Propanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
10.35
-
 methanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
17.38
-
 ethanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
36.83
-
 methanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
51.24
-
 methanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, strong activation Lysinibacillus xylanilyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
420000
-
 gel filtration Lysinibacillus xylanilyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + NAD+ Lysinibacillus xylanilyticus
-
 formaldehyde + NADH + H+
-
 ?
methanol + NAD+ Lysinibacillus xylanilyticus KCTC 13423
-
 formaldehyde + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Lysinibacillus xylanilyticus
-
-
-
Lysinibacillus xylanilyticus KCTC 13423
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus xylanilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanol + NAD+
-
 Lysinibacillus xylanilyticus butyraldehyde + NADH + H+
-
 ?
butanol + NAD+
-
 Lysinibacillus xylanilyticus KCTC 13423 butyraldehyde + NADH + H+
-
 ?
ethanol + NAD+
-
 Lysinibacillus xylanilyticus acetaldehyde + NADH + H+
-
 ?
ethanol + NAD+
-
 Lysinibacillus xylanilyticus KCTC 13423 acetaldehyde + NADH + H+
-
 ?
methanol + NAD+
-
 Lysinibacillus xylanilyticus formaldehyde + NADH + H+
-
 ?
methanol + NAD+
-
 Lysinibacillus xylanilyticus KCTC 13423 formaldehyde + NADH + H+
-
 ?
n-propanol + NAD+
-
 Lysinibacillus xylanilyticus propionaldehyde + NADH + H+
-
 ?
n-propanol + NAD+
-
 Lysinibacillus xylanilyticus KCTC 13423 propionaldehyde + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
decamer 10 * 42800, SDS-PAGE Lysinibacillus xylanilyticus

Synonyms

Synonyms Comment Organism
lxmdh
-
 Lysinibacillus xylanilyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 in the presence of 10 mM Mg2+ Lysinibacillus xylanilyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 35°C: about 40% of maximal activity, 70°C: about 50% of maximal activity Lysinibacillus xylanilyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
 methanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.24
-
 methanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
0.31
-
 n-Propanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
0.33
-
 methanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
0.33
-
 methanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
0.37
-
 NAD+ pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.46
-
 ethanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.52
-
 butanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.55
-
 n-Propanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.59
-
 ethanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
0.62
-
 ethanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
0.65
-
 ethanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
0.69
-
 n-Propanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
0.79
-
 n-Propanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 in the presence of 10 mM Mg2+ Lysinibacillus xylanilyticus

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 pH 8.5: about 50% of maximal activity, pH 10.0: about 90% of maximal activity Lysinibacillus xylanilyticus

General Information

General Information Comment Organism
metabolism methanol oxidation catalyzed by methanol dehydrogenase is one of the key steps in methanol utilization in bacterial methylotrophy Lysinibacillus xylanilyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0064
-
 methanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
0.0092
-
 methanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
0.0236
-
 methanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
0.0335
-
 n-Propanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
0.0339
-
 ethanol pH 9.5, 55°C, mutant enzyme S101V Lysinibacillus xylanilyticus
0.0668
-
 methanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.34
-
 n-Propanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
0.381
-
 n-Propanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
0.474
-
 ethanol pH 9.5, 55°C, mutant enzyme T141S Lysinibacillus xylanilyticus
0.509
-
 n-Propanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
0.523
-
 ethanol pH 9.5, 55°C, mutant enzyme A164F Lysinibacillus xylanilyticus
1.048
-
 butanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
1.648
-
 NAD+ pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus
1.861
-
 ethanol pH 9.5, 55°C, wild-type enzyme Lysinibacillus xylanilyticus