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Literature summary for 1.1.1.215 extracted from

  • Ameyama, M.; Adachi, O.
    2-keto-D-Gluconate reductase from acetic acid bacteria (1982), Methods Enzymol., 89, 203-210.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Acetobacter pasteurianus
-
Gluconacetobacter liquefaciens
-
Acetobacter ascendens

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition Acetobacter ascendens
Hg2+ complete inhibition Acetobacter pasteurianus
Hg2+ complete inhibition Gluconacetobacter liquefaciens
Hg2+ complete inhibition Gluconobacter oxydans
sulfhydryl reagents strong Acetobacter ascendens
sulfhydryl reagents strong Acetobacter pasteurianus
sulfhydryl reagents strong Gluconacetobacter liquefaciens
sulfhydryl reagents strong Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Gluconobacter oxydans 5737
-
cytoplasm
-
Acetobacter pasteurianus 5737
-
cytoplasm
-
Gluconacetobacter liquefaciens 5737
-
cytoplasm
-
Acetobacter ascendens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal requirement Gluconobacter oxydans
additional information no metal requirement Acetobacter pasteurianus
additional information no metal requirement Gluconacetobacter liquefaciens
additional information no metal requirement Acetobacter ascendens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
2 * 43000 + 1 * 34000, SDS-PAGE Gluconacetobacter liquefaciens
43000
-
2 * 43000 + 1 * 34000, SDS-PAGE Gluconacetobacter liquefaciens
120000
-
gel filtration Acetobacter pasteurianus
120000
-
gel filtration Gluconacetobacter liquefaciens
120000
-
gel filtration Acetobacter ascendens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydro-D-gluconate + NADPH Acetobacter pasteurianus
-
D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH Gluconobacter oxydans reverse reaction at the same rate by Gluconobacter enzyme D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH Gluconacetobacter liquefaciens reverse reaction at the same rate by Gluconobacter enzyme D-gluconate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Acetobacter ascendens
-
-
-
Acetobacter pasteurianus
-
-
-
Gluconacetobacter liquefaciens
-
-
-
Gluconobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconacetobacter liquefaciens
affinity chromatography on Blue-Dextran Sepharose B Acetobacter ascendens
partial Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
61.03
-
-
Gluconacetobacter liquefaciens
187
-
-
Acetobacter ascendens
336
-
-
Acetobacter pasteurianus

Storage Stability

Storage Stability Organism
5┬░C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Gluconobacter oxydans
5┬░C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Acetobacter pasteurianus
5┬░C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Gluconacetobacter liquefaciens
5┬░C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol Acetobacter ascendens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-D-gluconate + NADPH
-
Acetobacter pasteurianus D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Gluconobacter oxydans D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Gluconacetobacter liquefaciens D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Acetobacter ascendens D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH strongly preferred reaction of Acetobacter enzyme Acetobacter pasteurianus D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH strongly preferred reaction of Acetobacter enzyme Acetobacter ascendens D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH reverse reaction at the same rate by Gluconobacter enzyme Gluconobacter oxydans D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH reverse reaction at the same rate by Gluconobacter enzyme Gluconacetobacter liquefaciens D-gluconate + NADP+
-
r
2-keto-L-gulonate + NADPH third best substrate Gluconobacter oxydans L-idonate + NADP+
-
r
2-keto-L-gulonate + NADPH third best substrate Gluconacetobacter liquefaciens L-idonate + NADP+
-
r
2-keto-L-gulonate + NADPH third best substrate Acetobacter ascendens L-idonate + NADP+
-
r
2-keto-L-gulonate + NADPH betst substrate Acetobacter pasteurianus L-idonate + NADP+
-
r
5-keto-D-gluconate + NADPH
-
Gluconobacter oxydans D-gluconate + NADP+
-
?
5-keto-D-gluconate + NADPH
-
Gluconacetobacter liquefaciens D-gluconate + NADP+
-
?
5-keto-D-gluconate + NADPH poor substrate Acetobacter pasteurianus D-gluconate + NADP+
-
?
5-keto-D-gluconate + NADPH poor substrate Acetobacter ascendens D-gluconate + NADP+
-
?
D-galactonate + NADP+
-
Acetobacter pasteurianus 2-dehydro-D-galactonate + NADPH
-
r
D-galactonate + NADP+
-
Acetobacter ascendens 2-dehydro-D-galactonate + NADPH
-
r
D-galactonate + NADP+ third best substrate Gluconobacter oxydans 2-dehydro-D-galactonate + NADPH
-
r
D-galactonate + NADP+ third best substrate Gluconacetobacter liquefaciens 2-dehydro-D-galactonate + NADPH
-
r
D-xylonate + NADPH
-
Gluconobacter oxydans 2-keto-D-xylonate + NADP+
-
?
D-xylonate + NADPH
-
Acetobacter pasteurianus 2-keto-D-xylonate + NADP+
-
?
D-xylonate + NADPH
-
Gluconacetobacter liquefaciens 2-keto-D-xylonate + NADP+
-
?
L-idonate + NADP+ poor substrate Acetobacter pasteurianus 2-keto-L-idonate + NADPH
-
?
L-idonate + NADP+ poor substrate Acetobacter ascendens 2-keto-L-idonate + NADPH
-
?
L-idonate + NADP+ best substrate Gluconobacter oxydans 2-keto-L-idonate + NADPH
-
?
L-idonate + NADP+ best substrate Gluconacetobacter liquefaciens 2-keto-L-idonate + NADPH
-
?
additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Gluconobacter oxydans ?
-
?
additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Acetobacter pasteurianus ?
-
?
additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Gluconacetobacter liquefaciens ?
-
?
additional information no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate Acetobacter ascendens ?
-
?

Subunits

Subunits Comment Organism
trimer
-
Acetobacter ascendens
trimer 2 * 43000 + 1 * 34000, SDS-PAGE Gluconacetobacter liquefaciens

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
55
-
stable for 5 min, enhanced by the addition of substrate Gluconobacter oxydans
55
-
stable for 5 min, enhanced by the addition of substrate Acetobacter pasteurianus
55
-
stable for 5 min, enhanced by the addition of substrate Gluconacetobacter liquefaciens
55
-
stable for 5 min, enhanced by the addition of substrate Acetobacter ascendens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of 2-ketogluconate Gluconobacter oxydans
6
-
reduction of 2-ketogluconate Acetobacter pasteurianus
6
-
reduction of 2-ketogluconate Gluconacetobacter liquefaciens
6
-
reduction of 2-ketogluconate Acetobacter ascendens
10.5
-
-
Acetobacter ascendens
10.5
-
oxidation of gluconate Gluconacetobacter liquefaciens
11
-
oxidation of gluconate Acetobacter ascendens
12
-
oxidation of gluconate Acetobacter pasteurianus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5┬░C Gluconobacter oxydans
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5┬░C Acetobacter pasteurianus
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5┬░C Gluconacetobacter liquefaciens
6
-
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5┬░C Acetobacter ascendens

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Gluconobacter oxydans
additional information no activity with NADH Acetobacter pasteurianus
additional information no activity with NADH Gluconacetobacter liquefaciens
additional information no activity with NADH Acetobacter ascendens
NADP+
-
Gluconobacter oxydans
NADP+
-
Acetobacter pasteurianus
NADP+
-
Gluconacetobacter liquefaciens
NADP+
-
Acetobacter ascendens
NADPH
-
Gluconobacter oxydans
NADPH
-
Acetobacter pasteurianus
NADPH
-
Gluconacetobacter liquefaciens
NADPH
-
Acetobacter ascendens