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Literature summary for 1.1.1.215 extracted from

  • Adachi, O.; Chiyonobu, T.; Shinagawa, E.; Matsushita, K.; Ameyama, M.
    Crystalline 2-ketogluconate reductase from Acetobacter ascendens, the second instance of crystalline enzyme in genus Acetobacter (1978), Agric. Biol. Chem., 42, 2057-2062.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Acetobacter ascendens

General Stability

General Stability Organism
2-ketogluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l Acetobacter ascendens
5-ketogluconate does not stabilize Acetobacter ascendens
gluconate enhances thermal and storage stability even of dilute enzyme solutions, 0.1 mg protein/l Acetobacter ascendens

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition Acetobacter ascendens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0074
-
NADPH
-
Acetobacter ascendens
1.2
-
NADP+
-
Acetobacter ascendens
4.4
-
gluconate
-
Acetobacter ascendens
5.3
-
2-keto-D-gluconate
-
Acetobacter ascendens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Acetobacter ascendens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal requirement Acetobacter ascendens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
3 * 40000, SDS-PAGE Acetobacter ascendens
120000
-
gel filtration Acetobacter ascendens

Organism

Organism UniProt Comment Textmining
Acetobacter ascendens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on Blue-Dextran Sepharose B Acetobacter ascendens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
187
-
-
Acetobacter ascendens

Storage Stability

Storage Stability Organism
ammonium sulfate solution of crystalline enzyme stable for over 2 years Acetobacter ascendens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydro-D-gluconate + NADPH i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter Acetobacter ascendens D-gluconate + NADP+
-
r
2-dehydro-D-gluconate + NADPH equal reaction rate of reduction of 2-ketogluconate and oxidation of gluconate with Gluconobacter enzyme Acetobacter ascendens D-gluconate + NADP+
-
r
2-keto-L-gulonate + NADPH
-
Acetobacter ascendens L-idonate + NADP+
-
r
5-keto-D-gluconate + NADPH poor substrate Acetobacter ascendens D-gluconate + NADP+
-
?
D-galactonate + NADP+
-
Acetobacter ascendens 2-dehydro-D-galactonate + NADPH
-
r
L-idonate + NADP+
-
Acetobacter ascendens 2-keto-L-idonate + NADPH
-
?
additional information D-xylonate, D-glucose, D-fructose, L-sorbose, 5-keto-D-fructose, D-sorbitol, glycerol Acetobacter ascendens ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 40000, SDS-PAGE Acetobacter ascendens

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
55
-
-
Acetobacter ascendens

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
35 65 about half-maximal activity at 35┬░C and 65┬░C Acetobacter ascendens

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
42
-
stable for 10 min, oxidation activity Acetobacter ascendens
53
-
stable for 10 min, reduction activity Acetobacter ascendens
70
-
stable for 10 min in the presence of either 2-ketogluconate or gluconate Acetobacter ascendens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of 2-ketogluconate Acetobacter ascendens
10.5
-
-
Acetobacter ascendens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 about half-maximal activity at pH 4.5 and 8.5, reduction of ketogluconate Acetobacter ascendens
10 12 about half-maximal activity at pH 10 and 12, oxidation of gluconate Acetobacter ascendens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Acetobacter ascendens
NADPH
-
Acetobacter ascendens