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Literature summary for 1.1.1.21 extracted from

  • Paidimuddala, B.; Krishna Aradhyam, G.; Gummadi, S.
    A halotolerant aldose reductase from Debaryomyces nepalensis gene isolation, overexpression and biochemical characterization (2017), RSC Adv., 7, 20384-20393 .
No PubMed abstract available
Search for more literature for 1.1.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Debaryomyces nepalensis

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure, a uniform negative electrostatic potential exists over the protein surface Debaryomyces nepalensis

Inhibitors

Inhibitors Comment Organism Structure
KCl 0.5 M, 76% residual activity, 1 M, 37% residual activity Debaryomyces nepalensis
NaCl 0.5 M, 73% residual activity, 1 M, 29% residual activity Debaryomyces nepalensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 NADPH pH 7.0, 45°C Debaryomyces nepalensis
1.7
-
 3-Nitrobenzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
2
-
 isophthalaldehyde pH 7.0, 45°C Debaryomyces nepalensis
3.3
-
 benzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
4.5
-
 Pyridine-3-aldehyde pH 7.0, 45°C Debaryomyces nepalensis
70
-
 Glyoxal pH 7.0, 45°C Debaryomyces nepalensis
106
-
 D-xylose pH 7.0, 45°C Debaryomyces nepalensis
110
-
 D-arabinose pH 7.0, 45°C Debaryomyces nepalensis
377
-
 D-ribose pH 7.0, 45°C Debaryomyces nepalensis
572
-
 D-galactose pH 7.0, 45°C Debaryomyces nepalensis

Organism

Organism UniProt Comment Textmining
Debaryomyces nepalensis A0A0M4HL56
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
 substrate D-xylose, pH 7.0, 45°C Debaryomyces nepalensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-nitrobenzaldehyde + NADPH + H+
-
 Debaryomyces nepalensis 3-nitrobenzyl alcohol + NADP+
-
 ?
benzaldehyde + NADPH + H+
-
 Debaryomyces nepalensis benzyl alcohol + NADP+
-
 ?
D-arabinose + NADPH + H+
-
 Debaryomyces nepalensis arabitol + NADP+
-
 ?
D-galactose + NADPH + H+
-
 Debaryomyces nepalensis D-galactitol + NADP+
-
 ?
D-ribose + NADPH + H+
-
 Debaryomyces nepalensis adonitol + NADP+
-
 ?
D-xylose + NADPH + H+
-
 Debaryomyces nepalensis D-xylitol + NADP+
-
 ?
glyoxal + NADPH + H+
-
 Debaryomyces nepalensis ?
-
 ?
isophthalaldehyde + NADPH + H+
-
 Debaryomyces nepalensis ?
-
 ?
additional information poor substrates: D-glucose, D-fructose. No substrate: D-mannose Debaryomyces nepalensis ?
-
-
pyridine-3-aldehyde + NADPH + H+
-
 Debaryomyces nepalensis 3-pyridinemethanol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 36700, calculated from sequence Debaryomyces nepalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Debaryomyces nepalensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 drastic loss of activity above Debaryomyces nepalensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 isophthalaldehyde pH 7.0, 45°C Debaryomyces nepalensis
7
-
 Pyridine-3-aldehyde pH 7.0, 45°C Debaryomyces nepalensis
11
-
 benzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
40
-
 3-Nitrobenzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
41
-
 Glyoxal pH 7.0, 45°C Debaryomyces nepalensis
92
-
 D-ribose pH 7.0, 45°C Debaryomyces nepalensis
106
-
 D-galactose pH 7.0, 45°C Debaryomyces nepalensis
108
-
 D-xylose pH 7.0, 45°C Debaryomyces nepalensis
163
-
 D-arabinose pH 7.0, 45°C Debaryomyces nepalensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Debaryomyces nepalensis

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 60% of maximum activity Debaryomyces nepalensis
8
-
 55 of maximum activity Debaryomyces nepalensis

Cofactor

Cofactor Comment Organism Structure
NADPH shows strict NADPH dependence Debaryomyces nepalensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 D-galactose pH 7.0, 45°C Debaryomyces nepalensis
0.24
-
 D-ribose pH 7.0, 45°C Debaryomyces nepalensis
0.58
-
 Glyoxal pH 7.0, 45°C Debaryomyces nepalensis
1
-
 D-xylose pH 7.0, 45°C Debaryomyces nepalensis
1.5
-
 D-arabinose pH 7.0, 45°C Debaryomyces nepalensis
1.6
-
 Pyridine-3-aldehyde pH 7.0, 45°C Debaryomyces nepalensis
3.3
-
 benzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
12
-
 isophthalaldehyde pH 7.0, 45°C Debaryomyces nepalensis
24
-
 3-Nitrobenzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
728
-
 NADPH pH 7.0, 45°C Debaryomyces nepalensis