Cloned (Comment) | Organism |
---|---|
heterologous expression in Escherichia coli | Eggerthella sp. CAG:298 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0114 | - |
12-oxo-deoxycholic acid | coenzyme: NADPH, pH 7.0, temperature not specified in the publication | Eggerthella sp. CAG:298 | |
0.1397 | - |
deoxycholic acid | coenzyme: NADP+, pH 7.0, temperature not specified in the publication | Eggerthella sp. CAG:298 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26355 | - |
calculation from sequence | Eggerthella sp. CAG:298 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eggerthella sp. CAG:298 | R7AM69 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Eggerthella sp. CAG:298 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
12-oxo-cholic acid + NAD(P)H + H+ | 4.1% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | cholic acid + NAD(P)+ | - |
r | |
12-oxo-deoxycholic acid + NAD(P)H + H+ | - |
Eggerthella sp. CAG:298 | deoxycholic acid + NAD(P)+ | - |
r | |
cholic acid + NAD(P)+ | 55.7% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | 12-oxocholic acid + NAD(P)H + H+ | - |
r | |
deoxycholic acid + NAD(P)+ | 51.9% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | 12-oxo-deoxycholic acid + NAD(P)H + H+ | - |
r | |
glycocholic acid + NAD(P)+ | 60.3% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | ? + NAD(P)H + H+ | - |
r | |
glycodeoxycholic acid + NAD(P)+ | 55.9% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | ? + NAD(P)H + H+ | - |
r | |
taurocholic acid + NAD(P)+ | 53.8% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | 2-[(3alpha,7alpha-dihydroxy-12,24-dioxo-5beta-cholan-24-yl)amino]ethane-1-sulfonic acid + NAD(P)H + H+ | - |
r | |
taurodeoxychloic acid + NAD(P)+ | 58.9% of the activity compared to the substrate 12-oxo-deoxycholic acid | Eggerthella sp. CAG:298 | ? + NAD(P)H + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 26355, calculation from sequence | Eggerthella sp. CAG:298 |
Synonyms | Comment | Organism |
---|---|---|
12alpha-HSDH | - |
Eggerthella sp. CAG:298 |
CDD59475 | - |
Eggerthella sp. CAG:298 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.63 | - |
12-oxo-deoxycholic acid | coenzyme: NADPH, pH 7.0, temperature not specified in the publication | Eggerthella sp. CAG:298 | |
10 | - |
deoxycholic acid | coenzyme: NADP+, pH 7.0, temperature not specified in the publication | Eggerthella sp. CAG:298 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
oxidative reaction and reductive reaction | Eggerthella sp. CAG:298 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Eggerthella sp. CAG:298 | |
NADPH | - |
Eggerthella sp. CAG:298 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme in an iso-bile acid pathway | Eggerthella sp. CAG:298 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
71.6 | - |
deoxycholic acid | coenzyme: NADP+, pH 7.0, temperature not specified in the publication | Eggerthella sp. CAG:298 | |
581.6 | - |
12-oxo-deoxycholic acid | coenzyme: NADPH, pH 7.0, temperature not specified in the publication | Eggerthella sp. CAG:298 |