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Information on EC 1.1.1.176 - 12alpha-hydroxysteroid dehydrogenase
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EC Tree
1.1.1.176 12alpha-hydroxysteroid dehydrogenaseIUBMB Comments
Catalyses the oxidation of the 12alpha-hydroxy group of bile acids, both in their free and conjugated form. Also acts on bile alcohols.
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Word Map
- 1.1.1.176
- bile
- synthesis
- clostridium
- sephadex
-
7alpha-oh
-
7alpha-hydroxysteroid
-
unconjugated
- dehydrogenases
-
7alpha
- degradation
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
12alpha-hydroxysteroid dehydrogenase, 12alpha-hsdh, nad-dependent 12alpha-hydroxysteroid dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
12alpha-HSDH
12alpha-hydroxy steroid dehydrogenase
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dehydrogenase, 12alpha-hydroxy steroid
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-
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Elen_2515
NAD+-dependent 12alpha-HSDH
NAD-dependent 12alpha-hydroxysteroid dehydrogenase
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NADP+-dependent 12alpha-HSDH
NADP-12alpha-hydroxysteroid dehydrogenase
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-
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cholate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate + NADPH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
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-
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SYSTEMATIC NAME
IUBMB Comments
12alpha-hydroxysteroid:NADP+ 12-oxidoreductase
Catalyses the oxidation of the 12alpha-hydroxy group of bile acids, both in their free and conjugated form. Also acts on bile alcohols.
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CAS REGISTRY NUMBER
COMMENTARY
61642-40-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
12-oxo-cholic acid + NAD(P)H + H+
cholic acid + NAD(P)+
4.1% of the activity compared to the substrate 12-oxo-deoxycholic acid
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-
r
12-oxo-deoxycholic acid + NAD(P)H + H+
deoxycholic acid + NAD(P)+
-
-
-
r
12-oxochenodeoxycholic acid + NADP+
(3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid + NADPH + H+
-
-
-
-
?
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl methyl ester + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl methyl ester + NADPH + H+
-
-
-
-
r
5beta-cholanic acid-3,7,12-trione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
7alpha,12alpha-dihydroxy-5beta-cholanoate + NAD+
7alpha-hydroxy-12-oxo-5beta-cholanoate + NADH
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-
-
-
?
allocholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
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-
-
-
?
allodeoxycholate + NAD+
3alpha-hydroxy-12-oxo-5alpha-cholan-24-oic acid + NADH
-
-
-
-
?
cholic acid + NAD(P)+
12-oxocholic acid + NAD(P)H + H+
55.7% of the activity compared to the substrate 12-oxo-deoxycholic acid
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-
r
cholic acid + NADP+
12-ketochenodeoxycholic acid + NADPH + H+
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the assay is performed using an NADP+-regenerating system with acetone, NADPH, 2-propanol and alcohol dehydrogenase from Lactobacillus brevis or Thermoanaerobium brockii, overview
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r
deoxycholic acid + NAD(P)+
12-oxo-deoxycholic acid + NAD(P)H + H+
51.9% of the activity compared to the substrate 12-oxo-deoxycholic acid
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-
r
deoxycholic acid + NAD+
12-oxolithocholic acid + NADH + H+
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100% activity
-
-
r
deoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NADH + H+
-
-
-
-
?
deoxycholic acid + NADP+
12-oxolithocholic acid + NADH + H+
-
3.14% activity compared to NAD+
-
-
r
glycocholic acid + NAD(P)+
? + NAD(P)H + H+
60.3% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
glycodeoxycholic acid + NAD(P)+
? + NAD(P)H + H+
55.9% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
glycodeoxycholic acid + NAD+
? + NADH + H+
-
97.8% activity compared to deoxycholic acid
-
-
r
taurocholic acid + NAD(P)+
2-[(3alpha,7alpha-dihydroxy-12,24-dioxo-5beta-cholan-24-yl)amino]ethane-1-sulfonic acid + NAD(P)H + H+
53.8% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
taurocholic acid + NAD+
? + NADH + H+
-
98.95% activity compared to deoxycholic acid
-
-
r
taurodeoxychloic acid + NAD(P)+
? + NAD(P)H + H+
58.9% of the activity compared to the substrate 12-oxo-deoxycholic acid
-
-
r
taurodeoxycholic acid + NAD+
? + NADH + H+
-
90.84% activity compared to deoxycholic acid
-
-
r
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
3.65% activity compared to 12-oxolithocholic acid
-
-
?
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
3.65% activity compared to 12-oxolithocholic acid
-
-
?
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
4.16% activity compared to 12-oxolithocholic acid
-
-
?
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
4.16% activity compared to 12-oxolithocholic acid
-
-
?
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
-
9.55% activity compared to 12-oxolithocholic acid
-
-
?
12-oxochenodeoxycholic acid + NADPH + H+
7-oxodeoxycholic acid + NADP+
-
9.55% activity compared to 12-oxolithocholic acid
-
-
?
12-oxolithocholic acid + NADH + H+
deoxycholic acid + NAD+
-
19.6% activity compared to deoxycholic acid
-
-
?
12-oxolithocholic acid + NADH + H+
deoxycholic acid + NAD+
Eggerthella lenta C592
-
19.6% activity compared to deoxycholic acid
-
-
?
12-oxolithocholic acid + NADH + H+
deoxycholic acid + NAD+
-
-
-
-
r
12-oxolithocholic acid + NADH + H+
deoxycholic acid + NAD+
-
19.6% activity compared to deoxycholic acid
-
-
?
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
100% activity
-
-
?
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
100% activity
-
-
?
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
100% activity
-
-
?
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
100% activity
-
-
?
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
-
100% activity
-
-
?
12-oxolithocholic acid + NADPH + H+
deoxycholic acid + NADP+
-
100% activity
-
-
?
3-oxocholic acid + NADPH + H+
cholic acid + NADP+
-
-
-
r
3-oxocholic acid + NADPH + H+
cholic acid + NADP+
-
-
-
r
3-oxocholic acid + NADPH + H+
cholic acid + NADP+
-
-
-
-
r
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
-
-
-
-
?
5beta-cholanic acid-3,12-dione + NADPH + H+
3-oxodeoxycholic acid + NADP+
-
-
-
-
?
5beta-cholanic acid-3,7,12-trione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3,7,12-trione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3,7,12-trione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
-
-
-
-
?
5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
-
-
-
?
5beta-cholanic acid-3alpha-ol-7,12-dione + NADPH + H+
cholic acid + deoxycholic acid + 3-oxodeoxycholic acid + NADP+
-
-
-
-
?
7-oxocholic acid + NADPH + H+
deoxycholic acid + NADP+
-
-
-
r
cholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
-
-
-
?
cholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
conversion of CA into 12-oxoursodeoxycholic acid
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
conversion of CA into 12-oxoursodeoxycholic acid
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
conversion of CA into 12-oxoursodeoxycholic acid
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
conversion of CA into 12-oxoursodeoxycholic acid
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
conversion of CA into 12-oxoursodeoxycholic acid
-
-
?
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
?
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
-
r
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
-
?
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
hardly accepts N6-modified NADP+ derivative
-
-
?, r
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid
-
-
?, r
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
-
?
cholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
-
-
-
?
cholic acid + NAD+
3-oxocholic acid + NADH + H+
-
98.5% activity compared to deoxycholic acid
-
-
r
cholic acid + NAD+
3-oxocholic acid + NADH + H+
Eggerthella lenta C592
-
98.5% activity compared to deoxycholic acid
-
-
r
cholic acid + NAD+
3-oxocholic acid + NADH + H+
-
98.5% activity compared to deoxycholic acid
-
-
r
cholic acid + NADP+
3-oxocholic acid + NADPH + H+
82.32% activity compared to deoxycholic acid
-
-
r
cholic acid + NADP+
3-oxocholic acid + NADPH + H+
80.51% activity compared to deoxycholic acid
-
-
r
cholic acid + NADP+
3-oxocholic acid + NADPH + H+
-
54.44% activity compared to deoxycholic acid
-
-
r
deoxycholate + NADP+
12-oxo-lithocholic acid + NADPH + H+
-
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
i.e. 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid
-
?
deoxycholate + NADP+
12-oxo-lithocholic acid + NADPH + H+
-
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
i.e. 3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid
-
?
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
r
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
deoxycholic acid + NAD(P)+
3alpha-hydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
deoxycholic acid + NADP+
7-oxocholic acid + NADPH + H+
100% activity
-
-
r
deoxycholic acid + NADP+
7-oxocholic acid + NADPH + H+
100% activity
-
-
?
deoxycholic acid + NADP+
7-oxocholic acid + NADPH + H+
-
100% activity
-
-
r
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
-
?
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
-
?
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
-
?
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
81.08% activity compared to deoxycholic acid
-
-
r
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
Eggerthella lenta C592
-
81.08% activity compared to deoxycholic acid
-
-
r
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
81.08% activity compared to deoxycholic acid
-
-
r
glycocholic acid + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine
-
-
?
glycodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
-
?
glycodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
-
?
glycodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
-
?
glycodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
i.e. 3alpha,12alpha-dihydroxy-5beta-cholanoyl glycine
-
-
?
glycodeoxycholic acid + NADP+
? + NADPH + H+
25.85% activity compared to deoxycholic acid
-
-
r
glycodeoxycholic acid + NADP+
? + NADPH + H+
24.17% activity compared to deoxycholic acid
-
-
r
glycodeoxycholic acid + NADP+
? + NADPH + H+
-
6.36% activity compared to deoxycholic acid
-
-
r
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine
-
-
?
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine
-
-
?
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine
-
-
?
taurocholic acid + NAD(P)+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl taurine + NAD(P)H
-
-
-
-
?
taurodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
-
-
-
-
?
taurodeoxycholic acid + NAD+
3alpha-hydroxy-12-oxo-5beta-cholanoyl taurine + NADH
-
-
-
-
?
taurodeoxycholic acid + NADP+
? + NADPH + H+
40.33% activity compared to deoxycholic acid
-
-
r
taurodeoxycholic acid + NADP+
? + NADPH + H+
45.08% activity compared to deoxycholic acid
-
-
r
taurodeoxycholic acid + NADP+
? + NADPH + H+
-
6.05% activity compared to deoxycholic acid
-
-
r
additional information
?
-
-
multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum
-
-
?
additional information
?
-
-
multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum
-
-
?
additional information
?
-
-
reaction product analysis confirmed by MS and NMR analyses
-
-
?
additional information
?
-
-
reaction product analysis confirmed by MS and NMR analyses
-
-
?
additional information
?
-
-
no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid
-
-
-
additional information
?
-
Eggerthella lenta C592
-
no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid
-
-
-
additional information
?
-
-
no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid
-
-
-
additional information
?
-
no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3'- or C-7'-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH
-
-
-
additional information
?
-
no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3'- or C-7'-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH
-
-
-
additional information
?
-
no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one and 7-oxocholic acid. No activity with NADH
-
-
-
additional information
?
-
no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one and 7-oxocholic acid. No activity with NADH
-
-
-
additional information
?
-
-
no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH
-
-
-
additional information
?
-
-
no detection of the formation of 5beta-cholanic acid-3alpha-ol-12-one when cholic acid or its C-3- or C-7-oxo derivative is the substrate. No detection of oxidation of the 12alpha-hydroxyl group when deoxycholic acid is the substrate. No activity with 5beta-cholanic acid-12alpha-ol-3-one. No activity with NADH
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
12-oxolithocholic acid + NADH + H+
deoxycholic acid + NAD+
-
-
-
-
r
cholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
-
-
-
?
cholate + NAD+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
-
-
-
?
cholate + NADP+
3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
-
-
-
-
?
additional information
?
-
-
multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum
-
-
?
additional information
?
-
-
multistep conversion of cholic acid into ursodeoxycholic acid. Enzyme sources are 7alpha-HSDH from Clostridium absonum (crude extract), 12alpha-HSDH from Clostridium group P, 7beta-HSDH from Clostridium absonum
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no substrate inhibition at high concentration of cholic acid
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE