Cloned (Comment) | Organism |
---|---|
- |
Flavobacterium frigidimaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 38% inhibition | Flavobacterium frigidimaris | |
CuCl2 | 1 mM, 68% inhibition | Flavobacterium frigidimaris | |
FeCl2 | 1 mM, 57% inhibition | Flavobacterium frigidimaris | |
hexadecyltrimethyl-ammonium bromide | 1 mM, 79% inhibition | Flavobacterium frigidimaris | |
HgCl2 | 1 mM, complete inhibition | Flavobacterium frigidimaris | |
Hydroxylamine hydrochloride | 1 mM, 34% inhibition | Flavobacterium frigidimaris | |
iodoacetic acid | 1 mM, 96% inhibition | Flavobacterium frigidimaris | |
NEM | 1 mM, complete inhibition | Flavobacterium frigidimaris | |
NiCl2 | 1 mM, 79% inhibition | Flavobacterium frigidimaris | |
PCMB | 1 mM, complete inhibition | Flavobacterium frigidimaris | |
polyoxyethylene octylphenyl ether | 1 mM, 43% inhibition | Flavobacterium frigidimaris | |
SDS | 1 mM, 91% inhibition | Flavobacterium frigidimaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.147 | - |
ethanol | 20°C, pH 9.0 | Flavobacterium frigidimaris | |
0.168 | - |
ethanol | 30°C, pH 9.0 | Flavobacterium frigidimaris | |
0.19 | - |
ethanol | 10°C, pH 9.0 | Flavobacterium frigidimaris | |
0.248 | - |
ethanol | 40°C, pH 9.0 | Flavobacterium frigidimaris | |
0.43 | - |
ethanol | 50°C, pH 9.0 | Flavobacterium frigidimaris | |
1.04 | - |
ethanol | 60°C, pH 9.0 | Flavobacterium frigidimaris | |
5.7 | - |
ethanol | 20°C, pH 9.0 | Saccharomyces cerevisiae | |
8.56 | - |
ethanol | 30°C, pH 9.0 | Saccharomyces cerevisiae | |
9.03 | - |
ethanol | 10°C, pH 9.0 | Saccharomyces cerevisiae | |
12.1 | - |
ethanol | 40°C, pH 9.0 | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn | the enzyme contains 2 gram-atoms Zn per subunit | Flavobacterium frigidimaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Flavobacterium frigidimaris |
160000 | - |
gel filtration | Flavobacterium frigidimaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium frigidimaris | Q8L3C9 | - |
- |
Flavobacterium frigidimaris KUC-1 | Q8L3C9 | - |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium frigidimaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Flavobacterium frigidimaris |
Storage Stability | Organism |
---|---|
-20°C, 10 mM potassium phosphate buffer, pH 7.0, containing 0.5 mM NAD+ without loss of activity for several months | Flavobacterium frigidimaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NAD+ | - |
Flavobacterium frigidimaris | butanal + NADH + H+ | - |
r | |
1-butanol + NAD+ | - |
Flavobacterium frigidimaris KUC-1 | butanal + NADH + H+ | - |
r | |
1-decanol + NAD+ | - |
Flavobacterium frigidimaris | decanal + NADH + H+ | - |
r | |
1-decanol + NAD+ | - |
Flavobacterium frigidimaris KUC-1 | decanal + NADH + H+ | - |
r | |
1-heptanol + NAD+ | - |
Flavobacterium frigidimaris | heptanal + NADH + H+ | - |
r | |
1-heptanol + NAD+ | - |
Flavobacterium frigidimaris KUC-1 | heptanal + NADH + H+ | - |
r | |
1-hexanol + NAD+ | - |
Flavobacterium frigidimaris | hexanal + NADH + H+ | - |
r | |
1-nonanol + NAD+ | - |
Flavobacterium frigidimaris | nonanal + NADH + H+ | - |
r | |
1-octanol + NAD+ | - |
Flavobacterium frigidimaris | octanal + NADH + H+ | - |
r | |
1-pentanol + NAD+ | - |
Flavobacterium frigidimaris | pentanal + NADH + H+ | - |
r | |
1-propanol + NAD+ | - |
Flavobacterium frigidimaris | propanal + NADH + H+ | - |
r | |
2-butanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-butanone + NADH + H+ | - |
r | |
2-decanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-decanone + NADH + H+ | - |
r | |
2-heptanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-heptanone + NADH + H+ | - |
r | |
2-hexanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-hexanone + NADH + H+ | - |
r | |
2-nonanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-nonanone + NADH + H+ | - |
r | |
2-octanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-octanone + NADH + H+ | - |
r | |
2-pentanol + NAD+ | weak activity | Flavobacterium frigidimaris | 2-pentanone + NADH + H+ | - |
r | |
2-propanol + NAD+ | - |
Flavobacterium frigidimaris | acetone + NADH + H+ | - |
r | |
2-propanol + NAD+ | - |
Flavobacterium frigidimaris KUC-1 | acetone + NADH + H+ | - |
r | |
allylalcohol + NAD+ | - |
Flavobacterium frigidimaris | prop-2-enal + NADH + H+ | - |
? | |
benzylalcohol + NAD+ | weak activity | Flavobacterium frigidimaris | benzaldehyde + NADH + H+ | - |
r | |
ethanol + NAD+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADH + H+ | - |
r | |
ethanol + NAD+ | - |
Flavobacterium frigidimaris | acetaldehyde + NADH + H+ | - |
r | |
additional information | no activity with methanol | Flavobacterium frigidimaris | ? | - |
? | |
additional information | no activity with methanol | Flavobacterium frigidimaris KUC-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Flavobacterium frigidimaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Saccharomyces cerevisiae |
70 | - |
- |
Flavobacterium frigidimaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
60 | 0°C: about 70% of maximal activity, about 45% of maximal activity | Flavobacterium frigidimaris |
20 | 85 | 20°C: about 65% of maximal activity, 85°C: about 90% of maximal activity | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 304 min in absence of NAD+ | Flavobacterium frigidimaris |
60 | - |
half-life: 2 min in absence of NAD+ | Saccharomyces cerevisiae |
60 | - |
half-life: 50 min in absence of NAD+, 143 min in presence of 0.01 mM NAD+ | Flavobacterium frigidimaris |
65 | - |
half-life: 20 min in absence of NAD+ | Flavobacterium frigidimaris |
70 | - |
half-life: 6 min in absence of NAD+ | Flavobacterium frigidimaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
39.9 | - |
ethanol | 10°C, pH 9.0 | Flavobacterium frigidimaris | |
62.48 | - |
ethanol | 20°C, pH 9.0 | Flavobacterium frigidimaris | |
109.5 | - |
ethanol | 30°C, pH 9.0 | Flavobacterium frigidimaris | |
160.8 | - |
ethanol | 40°C, pH 9.0 | Flavobacterium frigidimaris | |
251.3 | - |
ethanol | 10°C, pH 9.0 | Saccharomyces cerevisiae | |
262.3 | - |
ethanol | 50°C, pH 9.0 | Flavobacterium frigidimaris | |
432.3 | - |
ethanol | 20°C, pH 9.0 | Saccharomyces cerevisiae | |
474.9 | - |
ethanol | 60°C, pH 9.0 | Flavobacterium frigidimaris | |
786.1 | - |
ethanol | 30°C, pH 9.0 | Saccharomyces cerevisiae | |
895.4 | - |
ethanol | 40°C, pH 9.0 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of acetaldehyde | Flavobacterium frigidimaris |
6.7 | 7 | oxidation of ethanol | Flavobacterium frigidimaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for NAD+, no activity with NADP+, pro-R stereospecificity for hydrogen transfer | Flavobacterium frigidimaris |