Any feedback?
Information on EC 3.5.4.43 - hydroxydechloroatrazine ethylaminohydrolase
for references in articles please use BRENDA:EC3.5.4.43Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.5.4.43 hydroxydechloroatrazine ethylaminohydrolaseIUBMB Comments
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
adenosine aminohydrolase 3, hydroxyatrazine ethylaminohydrolase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosine aminohydrolase 3
AtzB
EC 3.5.99.3
-
-
formerly
-
FRAAL1473
FraEuI1c_5875
HAEA
hydroxyatrazine ethylaminohydrolase
Hydroxyatrazine hydrolase
-
-
-
-
additional information
-
the enzyme belongs to the amidohydrolase superfamily
AtzB
-
-
-
-
AtzB
Clavibacter michiganese ATZ1
-
-
-
HAEA
Clavibacter michiganese ATZ1
-
-
-
hydroxyatrazine ethylaminohydrolase
-
-
-
-
hydroxyatrazine ethylaminohydrolase
Clavibacter michiganese ATZ1
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hydroxyatrazine + H2O = N-isopropylammelide + ethylamine
Involved in a pathway by which the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, is degraded in bacteria via N-isopropylammelide, 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of C-N bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
hydroxyatrazine ethylaminohydrolase
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
188496-88-0
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
?
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-amino-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-azido-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-chloro-4-amino-6-hydroxy-s-triazine + H2O
4-amino-2,6-dihydroxy-s-triazine + HCl
-
-
ammelide is the sole reaction product
-
?
2-cyano-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-fluoro-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-hydroxy-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
?
-
-
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-ol + H2O
6-(propan-2-ylamino)-1,3,5-triazine-2,4-diol + ethanamine
-
-
-
-
ir
4-(N-ethylamino)-6-hydroxy-2-(N-methylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
4-amino-6-ethylamino-[1,3,5]triazine-2-ol + H2O
6-amino-[1,3,5]triazine-2,4-diol + ethylamine
-
-
-
-
?
4-amino-6-isopropylamino-[1,3,5]triazine-2-ol + H2O
6-amino-[1,3,5]triazine-2,4-diol + isopropylamine
-
-
-
-
?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
?
-
-
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
?
-
i.e. hydroxyatrazine
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
?
-
i.e. hydroxyatrazine
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
-
i.e. hydroxyatrazine
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
-
i.e. hydroxyatrazine
-
?
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
-
-
-
-
ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
Clavibacter michiganese ATZ1
-
-
-
-
ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
-
-
-
-
ir
additional information
?
-
-
the isolated strain is capable of degrading atrazine. Displacement of the three substituents on the s-triazine ring is mediated by three enzymatic steps encoded by the genes atzA, atzB, and atzC. AtzB catalyzes hydroxyatrazine deamidation, yielding N-isopropylammelide
-
-
?
additional information
?
-
-
the enzyme performs deamination and dechlorination, substrate specificity, overview
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-ol + H2O
6-(propan-2-ylamino)-1,3,5-triazine-2,4-diol + ethanamine
-
-
-
-
ir
additional information
?
-
-
the isolated strain is capable of degrading atrazine. Displacement of the three substituents on the s-triazine ring is mediated by three enzymatic steps encoded by the genes atzA, atzB, and atzC. AtzB catalyzes hydroxyatrazine deamidation, yielding N-isopropylammelide
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
?
-
i.e. hydroxyatrazine
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
?
-
i.e. hydroxyatrazine
-
-
?
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
-
-
-
-
ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
Clavibacter michiganese ATZ1
-
-
-
-
ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
-
-
-
-
ir
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isolated from Manfredi soil in Argentina used to grow corn and are treated with atrazine for weed control during 3 years, gene atzB
-
-
brenda
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Pseudoaminobacter sp.
-
-
brenda
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Nocardiodes sp.
-
-
brenda
use of atrazine as sole nitrogen source, but not as sole carbon source. Comparison of degradation products with those from Pseudoaminobacter sp. and Nocardiodes sp.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the enzyme is involved in degradation of the herbicide atrazine
physiological function
-
strain HB-6 is capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaves the s-triazine ring and mineralizes atrazine. In the growth medium, the amount of atrazine decreases rapidly, and hydroxyatrazine is observed to accumulate transiently and then undergo gradual degradation accompanied by the accumulation of cyanuric acid
physiological function
-
strain HB-6 is capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaves the s-triazine ring and mineralizes atrazine. In the growth medium, the amount of atrazine decreases rapidly, and hydroxyatrazine is observed to accumulate transiently and then undergo gradual degradation accompanied by the accumulation of cyanuric acid
-
Show AA Sequence and Transmembrane Helices (792 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme 6.6fold from Escherichia coli strain DH5alpha to homogeneity by ammonium sulfate fractionation, hydroxyapatite chromatography, and gel filtration
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene atzB, cloned from soil isolated strain, DNA and amino acid sequence determination and analysis, sequence comparison
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
exposure to atrazine results in up to 16fold increase in expression
expression of the atrazine degradation-related gene atzB in coculture treatments (of Arthrobacter sp. DNS10 and Enterobacter sp. P1 is a phosphorus-solubilizing bacterium that releases various kinds of organic acids but lacks the ability to degrade atrazine) is 1.8 times that of the single strain DNS10 culture treatment. This phenomenon is due to metabolite exchange between the two strains. Culturing these two strains together is a biostimulation strategy to enhance the biodegradation of atrazine by culturing these two strains together
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
degradation
agriculture
expression of the atrazine degradation-related gene atzB in coculture treatments (of Arthrobacter sp. DNS10 and Enterobacter sp. P1 is a phosphorus-solubilizing bacterium that releases various kinds of organic acids but lacks the ability to degrade atrazine) is 1.8 times that of the single strain DNS10 culture treatment. This phenomenon is due to metabolite exchange between the two strains. Culturing these two strains together is a biostimulation strategy to enhance the biodegradation of atrazine and the level of available phosphorus in soil by culturing these two strains together
degradation
-
strain ADP, use of atrazine as sole nitrogen source, but not as sole carbon source. Comparison of degradation products with those from Pseudoaminobacter sp. and Nocardiodes sp.
degradation
-
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Nocardiodes sp.
degradation
-
use of atrazine as sole nitrogen source and as sole carbon source. End product of atrazine metabolism is N-ethylammelide. Comparison of degradation products with those from Pseudomonas sp. and Nocardiodes sp.
degradation
-
mineralization of low concentrations of atrazine in the groundwater zone at low temperatures is possible by bioremediation treatments. In combined biostimulation treatment using citrate or molasses and augmentation with Pseudomonas citronellolis ADP or Arthrobacter aurescens strain TC1, up to 76% of atrazine is mineralized at 30°C, and the atrazine degradation gene numbers increase up to 10 million copies/g soil
degradation
mineralization of low concentrations of atrazine in the groundwater zone at low temperatures is possible by bioremediation treatments. In combined biostimulation treatment using citrate or molasses and augmentation with Pseudomonas citronellolis ADP or Arthrobacter aurescens strain TC1, up to 76%of atrazine is mineralized at 30°C, and the atrazine degradation gene numbers increase up to 10 million copies/g soil
degradation
-
strain HB-6 is capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaves the s-triazine ring and mineralizes atrazine. The strain demonstrate a very high efficiency of atrazine biodegradation with a broad optimum pH and temperature ranges and can be enhanced by cooperating with other bacteria
degradation
-
mineralization of low concentrations of atrazine in the groundwater zone at low temperatures is possible by bioremediation treatments. In combined biostimulation treatment using citrate or molasses and augmentation with Pseudomonas citronellolis ADP or Arthrobacter aurescens strain TC1, up to 76% of atrazine is mineralized at 30°C, and the atrazine degradation gene numbers increase up to 10 million copies/g soil
-
degradation
-
mineralization of low concentrations of atrazine in the groundwater zone at low temperatures is possible by bioremediation treatments. In combined biostimulation treatment using citrate or molasses and augmentation with Pseudomonas citronellolis ADP or Arthrobacter aurescens strain TC1, up to 76%of atrazine is mineralized at 30°C, and the atrazine degradation gene numbers increase up to 10 million copies/g soil
-
degradation
-
strain HB-6 is capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaves the s-triazine ring and mineralizes atrazine. The strain demonstrate a very high efficiency of atrazine biodegradation with a broad optimum pH and temperature ranges and can be enhanced by cooperating with other bacteria
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boundy-Mills, K.L.; de Souza, M.L.; Mandelbaum, R.T.; Wackett, L.P.; Sadowsky, M.J.
The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway
Appl. Environ. Microbiol.
63
916-923
1997
Pseudomonas sp.
brenda
Topp, E.; Zhu, H.; Nour, S.M.; Houhot, S.; Lewis, M.; Cuppels, D.
Characterization of an atrazine-degrading Pseudaminobacter sp. isolated from canadian and french agricultural soils
Appl. Environ. Microbiol.
66
2773-2787
2000
Aminobacter ciceronei
brenda
De Souza, M.L.; Seffernick, J.; Martinez, B.; Sadowsky, M.J.; Wackett, L.P.
The atrazine catabolism genes atzABC are widespread and highly conserved
J. Bacteriol.
180
1951-1954
1998
Pseudomonas sp.
brenda
Seffernick, J.L.; Shapir, N.; Schoeb, M.; Johnson, G.; Sadowsky, M.J.; Wackett, L.P.
Enzymatic degradation of chlorodiamino-s-triazine
Appl. Environ. Microbiol.
68
4672-4675
2002
Escherichia coli
brenda
Topp, E.
A comparison of three atrazine-degrading bacteria for soil bioremediation
Biol. Fertil. Soils
33
529-534
2001
Nocardioides sp., Pseudomonas sp., Pseudaminobacter sp.
brenda
Seffernick, J.L.; Aleem, A.; Osborne, J.P.; Johnson, G.; Sadowsky, M.J.; Wackett, L.P.
Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination
J. Bacteriol.
189
6989-6997
2007
Pseudomonas sp.
brenda
Govantes, F.; Garcia-Gonzalez, V.; Porrua, O.; Platero, A.I.; Jimenez-Fernandez, A.; Santero, E.
Regulation of the atrazine-degradative genes in Pseudomonas sp. strain ADP
FEMS Microbiol. Lett.
310
1-8
2010
Pseudomonas sp.
brenda
Govantes, F.; Porrua, O.; Garca-Gonzalez, V.; Santero, E.
Atrazine biodegradation in the lab and in the field: Enzymatic activities and gene regulation
Microb. Biotechnol.
2
178-185
2009
Enterobacter cloacae, Agrobacterium tumefaciens, Alcaligenes sp., Clavibacter michiganese, Pseudomonas sp., Cupriavidus basilensis, Pseudoaminobacter sp., Agrobacterium tumefaciens J14a, Alcaligenes sp. SG1, Cupriavidus basilensis M91-3, Enterobacter cloacae 99, Clavibacter michiganese ATZ1
brenda
Fernandez, L.; Valverde, C.; Gomez, M.
Isolation and characterization of atrazine-degrading Arthrobacter sp. strains from Argentine agricultural soils
Ann. Microbiol.
63
207-214
2013
Arthrobacter sp.
brenda
Rehan, M.; Kluge, M.; Fraenzle, S.; Kellner, H.; Ullrich, R.; Hofrichter, M.
Degradation of atrazine by Frankia alni ACN14a: gene regulation, dealkylation, and dechlorination
Appl. Microbiol. Biotechnol.
98
6125-6135
2014
Frankia sp. (E3IYB4), Frankia alni (Q0RQP4), Frankia sp. EuI1c (E3IYB4), Frankia alni ACN14a (Q0RQP4), Frankia alni ACN14a
brenda
Nousiainen, A.O.; Bjoerkloef, K.; Sagarkar, S.; Nielsen, J.L.; Kapley, A.; Jorgensen, K.S.
Bioremediation strategies for removal of residual atrazine in the boreal groundwater zone
Appl. Microbiol. Biotechnol.
99
10249-10259
2015
Pseudomonas citronellolis, Paenarthrobacter aurescens (A1RCX5), Pseudomonas citronellolis DSM 11735, Paenarthrobacter aurescens TC1 (A1RCX5)
brenda
Wang, J.; Zhu, L.; Wang, Q.; Wang, J.; Xie, H.
Isolation and characterization of atrazine mineralizing Bacillus subtilis strain HB-6
PLoS ONE
9
e107270
2014
Bacillus subtilis, Bacillus subtilis HB-6
brenda
Jiang, Z.; Zhang, X.; Wang, Z.; Cao, B.; Deng, S.; Bi, M.; Zhang, Y.
Enhanced biodegradation of atrazine by Arthrobacter sp. DNS10 during co-culture with a phosphorus solubilizing bacteria Enterobacter sp. P1
Ecotoxicol. Environ. Saf.
172
159-166
2019
Arthrobacter sp. DNS10 (T1Z2L1)
-
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 3.5.4.43)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
