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Literature summary for 3.5.4.43 extracted from

  • Seffernick, J.L.; Aleem, A.; Osborne, J.P.; Johnson, G.; Sadowsky, M.J.; Wackett, L.P.
    Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination (2007), J. Bacteriol., 189, 6989-6997.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene atzB, expression in Escherichia coli strain DH5alpha Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 72% inhibition, not due to metal removal Pseudomonas sp.
8-Hydroxyquinoline-5-sulfonic acid
-
Pseudomonas sp.
Chelex-100 resin
-
Pseudomonas sp.
dipicolinic acid
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, recombinant AtzB, SDS-PAGE Pseudomonas sp.
105000
-
recombinant AtzB, gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
gene atzB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 6.6fold from Escherichia coli strain DH5alpha to homogeneity by ammonium sulfate fractionation, hydroxyapatite chromatography, and gel filtration Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.05
-
purified recombinant enzyme Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-di(N-sec-butylamino)-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-di(N-tert-butylamino)-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-diamino-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-dichloro-6-amino-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-amino-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-azido-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-chloro-4-amino-6-hydroxy-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-cyano-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-fluoro-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-hydroxy-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-isopropylamino-4-ethylamino-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
2-mercapto-4-isopropylamino-6-ethylamino-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
4-(N-ethylamino)-6-hydroxy-2-(N-methylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
-
Pseudomonas sp. ?
-
?
additional information the enzyme performs deamination and dechlorination, substrate specificity, overview Pseudomonas sp. ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52000, recombinant AtzB, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
AtzB
-
Pseudomonas sp.
hydroxyatrazine N-ethylaminohydrolase
-
Pseudomonas sp.
More the enzyme belongs to the amidohydrolase superfamily Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing, recombinant AtzB
-
5
Pseudomonas sp. sequence calculation
-
5.7