Cloned (Comment) | Organism |
---|---|
gene atzB, expression in Escherichia coli strain DH5alpha | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 72% inhibition, not due to metal removal | Pseudomonas sp. | |
8-Hydroxyquinoline-5-sulfonic acid | - |
Pseudomonas sp. | |
Chelex-100 resin | - |
Pseudomonas sp. | |
dipicolinic acid | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, recombinant AtzB, SDS-PAGE | Pseudomonas sp. |
105000 | - |
recombinant AtzB, gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
gene atzB | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 6.6fold from Escherichia coli strain DH5alpha to homogeneity by ammonium sulfate fractionation, hydroxyapatite chromatography, and gel filtration | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.05 | - |
purified recombinant enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-di(N-sec-butylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-di(N-tert-butylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-diamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-dichloro-6-amino-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-amino-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-azido-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-chloro-4-amino-6-hydroxy-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-cyano-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-fluoro-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-hydroxy-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-isopropylamino-4-ethylamino-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
2-mercapto-4-isopropylamino-6-ethylamino-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
4-(N-ethylamino)-6-hydroxy-2-(N-methylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O | - |
Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme performs deamination and dechlorination, substrate specificity, overview | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, recombinant AtzB, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
AtzB | - |
Pseudomonas sp. |
hydroxyatrazine N-ethylaminohydrolase | - |
Pseudomonas sp. |
More | the enzyme belongs to the amidohydrolase superfamily | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing, recombinant AtzB | - |
5 |
Pseudomonas sp. | sequence calculation | - |
5.7 |