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IUBMB CommentsThe enzyme from Alcaligenes xylosoxydans subsp. xylosoxydans is specific for N-acyl-D-aspartate. Requires zinc.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
n-acyl-d-aspartate amidohydrolase, aminoacylase ii, n-acetyl-l-aspartate amidohydrolase, acylase ii,
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Acetylaspartic deaminase
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EC 3.5.1.15
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related
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N-Acetyl-L-aspartate amidohydrolase
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N-Acetylaspartate amidohydrolase
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N-Acyl-D-aspartate amidohydrolase
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N-acyl-D-aspartate + H2O = a carboxylate + D-aspartate
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hydrolysis of linear amides
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N-Acyl-D-aspartate amidohydrolase
The enzyme from Alcaligenes xylosoxydans subsp. xylosoxydans is specific for N-acyl-D-aspartate. Requires zinc.
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Gly-D-Asp + H2O
Glycine + Asp
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N-Acetyl-D-Asp + H2O
acetate + D-Asp
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N-Butanoyl-D-Asp + H2O
Butanoate + D-Asp
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N-Formyl-D-Asp + H2O
Formate + D-Asp
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N-glycyl-D-Asp + H2O
Gly + D-Asp
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N-Propanoyl-D-Asp + H2O
Propanoate + D-Asp
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additional information
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additional information
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no activity with N-carbobenzoyl-D-Asp, N-acetyl-L-Asp, N-acetyl-D-Glu
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additional information
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inducible by N-acetyl-D-Asp
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additional information
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inducible by N-acetyl-D-Asp
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Canavan Disease
Canavan disease and the role of N-acetylaspartate in myelin synthesis.
Canavan Disease
Purification, characterization, and localization of aspartoacylase from bovine brain.
Neurodegenerative Diseases
Canavan disease and the role of N-acetylaspartate in myelin synthesis.
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0.033
N-Butanoyl-D-Asp
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0.194
N-Propanoyl-D-Asp
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brenda
subsp. xylosoxydans strain A-6
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brenda
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53581
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1 * 53581, calculation from nucleotide sequence
54000
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1 * 54000, SDS-PAGE
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monomer
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1 * 54000, SDS-PAGE
monomer
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1 * 53581, calculation from nucleotide sequence
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10 min, stable up to
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10 min, 40% loss of activity
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expression in Escherichia coli
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Wakayama, M.; Watanabe, E.; Takenaka, Y.; Miyamoto, Y.; Tau, Y.; Sakai, K.; Moriguchi, M.
Cloning, expression, and nucleotide sequence of the N-acyl-D-aspartate amidohydrolase gene from Alcaligenes xylosoxydans subsp. xylosoxydans A-6
J. Ferment. Bioeng.
80
311-317
1995
Achromobacter xylosoxidans
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brenda
Moriguchi, M.; Sakai, K.; Katsuno, Y.; Maki, T.; Wakayama, M.
Purification and characterization of novel N-acyl-D-aspartate amidohydrolase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6
Biosci. Biotechnol. Biochem.
57
1145-1148
1993
Achromobacter xylosoxidans
brenda
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3.5.1.83 N-Acyl-D-aspartate deacylase
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