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EC Tree
IUBMB Comments Also acts, but more slowly, on a wide range of other monophosphates.
Word Map
3.1.3.60
psychosomatic
payment
remuneration
financing
psychoses
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
pepp, pepase, phosphoenolpyruvate phosphatase, pep phosphatase,
more
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acid phosphatase
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distinct from PEP phosphatase, high activity towards phosphoenolpyruvate, in contrast to PEP phosphatase able to hydrolyze diphosphate
phosphatase, phosphoenolpyruvate
-
-
-
-
phosphoenolpyruvate phosphatase
additional information
-
phosphoenolpyruvate-specific alkaline phosphatase activity is due to cytosolic pyruvate kinase
PEPP
-
-
phosphoenolpyruvate phosphatase
-
-
phosphoenolpyruvate phosphatase
-
-
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phosphoenolpyruvate + H2O = pyruvate + phosphate
-
-
-
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hydrolysis of phosphoric ester
additional information
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PEP phosphatase increases as active Fe decreases
hydrolysis of phosphoric ester
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-
hydrolysis of phosphoric ester
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phosphoenolpyruvate phosphohydrolase
Also acts, but more slowly, on a wide range of other monophosphates.
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1-naphthyl-phosphate + H2O
1-naphthol + phosphate
-
-
-
-
?
2,3-diphosphoglycerate + H2O
?
-
-
-
-
?
2-phosphoglycerate + H2O
glycerate + phosphate
-
poor substrate
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
ADP-glucose + H2O
?
-
poor substrate
-
-
?
D-fructose 2,6-diphosphate + H2O
?
-
poor substrate
-
-
?
D-gluconate 6-phosphate + H2O
D-gluconate + phosphate
-
-
-
-
?
D-glucose 1-phosphate + H2O
?
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
D-ribose 5-phosphate + H2O
D-ribose + phosphate
-
poor substrate
-
-
?
diphosphate + H2O
2 phosphate
GDP + H2O
?
-
poor substrate
-
-
?
IDP + H2O
?
-
poor substrate
-
-
?
O-phospho-L-threonine + H2O
L-threonine + phosphate
-
-
-
-
?
O-phospho-L-tyrosine + H2O
L-tyrosine + phosphate
-
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
UDP + H2O
?
-
poor substrate
-
-
?
additional information
?
-
-
the enzyme seems to be required for fruit development
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
-
?
ADP + H2O
?
-
-
-
-
?
ADP + H2O
?
-
poor substrate
-
-
?
ATP + H2O
?
-
-
-
-
?
ATP + H2O
?
-
poor substrate
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
-
poor substrate
-
-
?
diphosphate + H2O
2 phosphate
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-
-
-
?
diphosphate + H2O
2 phosphate
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poor substrate
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
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bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
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bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
relatively unspecific
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?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
the enzyme is a bypass enzyme of glycolysis, pathway overview
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-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
highly specific
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
-
?
phytic acid + H2O
?
-
-
-
-
?
phytic acid + H2O
?
-
-
-
-
?
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phosphoenolpyruvate + H2O
pyruvate + phosphate
additional information
?
-
-
the enzyme seems to be required for fruit development
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
bypasses ADP-dependent pyruvate kinase reaction during extended periods of phosphate starvation
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
the enzyme is a bypass enzyme of glycolysis, pathway overview
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
?
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Ca2+
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1.4 fold activation at 4 mM
Co2+
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1.4 fold activation at 4 mM
Mg2+
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1.7 fold activation at 5 mM
Mg2+
-
1.7 fold activation at 4 mM
Mn2+
-
1.4 fold activation at 4 mM
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CuSO4
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complete inhibition at 4 mM
D-fructose 1,6-bisphosphate
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complete inhibition at 5 mM, competitive
FeCl3
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complete inhibition at 4 mM
glyceraldhyde-3-phosphate
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competitive
Hg2+
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competitive towards Mg2+
Lead acetate
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70% inhibition at 8 mM
methylene blue
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after irradiation
MgADP
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41% inhibition at 0.5 mM, competitive
Ni2+
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competitive towards Mg2+
p-chloromercuribenzoate
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reversible with excess of cysteine
Rose bengal
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after irradiation
ZnCl2
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complete inhibition at 4 mM
3-phosphoglycerate
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competitive; complete inhibition at 5 mM
3-phosphoglycerate
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competitive
Cu2+
-
-
Cu2+
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competitive towards Mg2+
molybdate
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complete inhibition at 0.1 mM, competitive
NaF
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uncompetitive
phosphate
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-
phosphate
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complete inhibition at 0.5 mM, competitive
Zn2+
-
-
Zn2+
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competitive towards Mg2+
additional information
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several more substances found to be inhibitory, no inhibition with N-ethylmaleimide
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additional information
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the enzyme is tolerant to long-term high NaCl salt stress, no effect by 150 mM NaCl over 10 days on cell suspension culture
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additional information
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the enzyme activity in roots is not correlated with oxalic acid exudation due to induction by aluminium toxicity or phosphorus deficiency
-
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additional information
-
the enzyme is tolerant to long-term high NaCl salt stress, no effect by 150 mM NaCl over 10 days on cell suspension culture
-
additional information
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the enzyme activity in roots is not correlated with oxalic acid exudation due to induction by aluminium toxicity or phosphorus deficiency
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Campylobacter Infections
Peptidase PepP is a novel virulence factor of Campylobacter jejuni contributing to murine campylobacteriosis.
COVID-19
Prone Position after Liberation from Prolonged Mechanical Ventilation in COVID-19 Respiratory Failure.
Infections
A differential fluorescence-based genetic screen identifies Listeria monocytogenes determinants required for intracellular replication.
phosphoenolpyruvate phosphatase deficiency
Peptidase PepP is a novel virulence factor of Campylobacter jejuni contributing to murine campylobacteriosis.
Placenta Accreta
Analysis of clinical features of 231 cases with pernicious placenta previa: A retrospective cohort study.
Respiratory Insufficiency
Prone Position after Liberation from Prolonged Mechanical Ventilation in COVID-19 Respiratory Failure.
Starvation
Phosphate Starvation Inducible ;Bypasses' of Adenylate and Phosphate Dependent Glycolytic Enzymes in Brassica nigra Suspension Cells.
Starvation
Purification and Characterization of a Phosphoenolpyruvate Phosphatase from Brassica nigra Suspension Cells.
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0.5
2,3 diphosphoglycerate
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25°C, pH 5.6
1.21
3-phosphoglycerate
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25°C, pH 5.6
0.57 - 1.6
4-nitrophenyl phosphate
0.59
D-gluconate 6-phosphate
-
25°C, pH 5.6
0.24
D-glucose 1-phosphate
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25°C, pH 5.6
1.47 - 2.01
D-glucose 6-phosphate
0.05 - 0.44
phosphoenolpyruvate
0.57
4-nitrophenyl phosphate
-
25°C, pH 5.6
1.6
4-nitrophenyl phosphate
-
25°C, pH 6.6
0.74
ADP
-
25°C, pH 5.6
0.5
ATP
-
25°C, pH 5.6
1.47
D-glucose 6-phosphate
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25°C, pH 6.6
2.01
D-glucose 6-phosphate
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25°C, pH 5.6
0.05
phosphoenolpyruvate
-
25°C, pH 5.6
0.08
phosphoenolpyruvate
-
-
0.14
phosphoenolpyruvate
-
30°C, pH 8.5
0.44
phosphoenolpyruvate
-
25°C, pH 6.6
0.82
phytic acid
-
25°C, pH 5.6
1.36
phytic acid
-
25°C, pH 6.6
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0.05 - 3.3
3-phosphoglycerate
5
ATP
-
competitive, 30°C, pH 8.5
4.5
citrate
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competitive, 30°C, pH 8.5
0.22
Cu2+
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competitive towards Mg2+, 30°C, pH 8.5
0.06
D-fructose 1,6-bisphosphate
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25°C, pH 5.6
0.003
molybdate
-
25°C, pH 5.6
3
oxalate
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competitive, 30°C, pH 8.5
0.085
phosphate
-
25°C, pH 5.6
6.3
succinate
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competitive, 30°C, pH 8.5
0.023
Zn2+
-
competitive towards Mg2+, 30°C, pH 8.5
0.05
3-phosphoglycerate
-
25°C, pH 5.6
3.3
3-phosphoglycerate
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competitive, 30°C, pH 8.5
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1.05
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Vmax, purified enzyme, 30°C, pH 8.5
1.4
-
purified enzyme, 30°C, pH 8.5
1225
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purified enzyme, 25°C, pH 5.6
380
-
purified enzyme, 25°C, pH 5.6
53
-
purified enzyme, 25°C, pH 6.6
additional information
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specific activity determined for all substrates
additional information
-
seedlings of Citrus sinensis (L.) Osbeck cv. Xuegan are irrigated with Mg-deficient or Mg-sufficient nutrient solution every other day for 12 weeks. (PEPP) activity slightly increased in roots. In contrast, Mg-deficient leaves show decreased PEPP activity
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7.5
-
assay at pH 7.5 in Tris-HCl, or at pH 5.6 in sodium acetate
5.6
-
-
5.6
-
assay at pH 7.5 in Tris-HCl, or at pH 5.6 in sodium acetate
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7.2 - 9.8
-
half maximum activity
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brenda
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brenda
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brenda
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brenda
two genotypes
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-
brenda
cv. Micro-Tom
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-
brenda
mung bean
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brenda
grapevine
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-
brenda
-
-
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brenda
black mustard
-
-
brenda
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from seedlings
brenda
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the enzyme shows highly increased activity about 35 days after flowering
brenda
-
-
brenda
-
-
brenda
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-
brenda
-
-
brenda
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petiole suspension cells
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
geminating bean
brenda
-
highest activity at 36 h of germination
brenda
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-
-
brenda
-
-
brenda
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malfunction
-
seedlings of Citrus sinensis (L.) Osbeck cv. Xuegan are irrigated with Mg-deficient or Mg-sufficient nutrient solution every other day for 12 weeks. (PEPP) activity slightly increased in roots. In contrast, Mg-deficient leaves show decreased PEPP activity
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PEPP_ALLCE
481
0
55970
Swiss-Prot
Secretory Pathway (Reliability: 1 )
PPDE_DEIRA
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
255
0
27936
Swiss-Prot
-
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52000
-
SDS-PAGE, degradation product with 42000 Da
240000
-
-
60000
-
SDS-PAGE
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monomer
-
1 * 56000, gel filtration, SDS-PAGE
monomer
-
1 * 60000, gel filtration, SDS-PAGE
tetramer
-
4 * 52000, gel filtration, SDS-PAGE
tetramer
-
4 * 60000, gel filtration, SDS-PAGE
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5.7 - 7.8
-
stable
646426
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55
-
4 min, 20% loss of activity
60
-
4 min, 30% loss of activity
50
-
stable up to 50°C
50
-
half-life: 6.8 min, pH 6.5, 2.4 min, pH 7.5, 1.8 min, pH 8.6
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-10°C, purified enzyme, 50 days, 50% loss of activity
-
-15-4°C, 50 days, no loss of activity
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-80°C, purified enzyme, 6 months, stable
-
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copurifies with pyruvate kinase activity
-
-
-
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Duff, S.M.G.; Lefebvre, D.D.; Plaxton, W.C.
Purification, characterization, and subcellular localization of an acid phosphatase from black mustard cell-suspension cultures: comparison with phosphoenolpyruvate phosphatase
Arch. Biochem. Biophys.
286
226-232
1991
Brassica nigra
brenda
Duff, S.M.G.; Lefebvre, D.D.; Plaxton, W.C.
Purification and characterization of a phosphoenolpyruvate phosphatase from Brassica nigra suspension cells
Plant Physiol.
90
734-741
1989
Brassica nigra
brenda
Malhotra, O.P.; Kayastha, A.M.
Chemical inactivation and active site groups of phosphoenolpyruvate-phosphatase from germinating mung beans (Vigna radiata)
Plant Sci.
65
161-170
1989
Vigna radiata
-
brenda
Malhotra, O.P.; Kayastha, A.M.
Isolation and characterization of phosphoenolpyruvate phosphatase from germinating mung beans (Vigna radiata)
Plant Physiol.
93
194-200
1990
Vigna radiata
brenda
Podesta, F.E.; Plaxton, W.C.
Association of phosphoenolpyruvate phosphatase activity with the cytosolic pyruvate kinase of germinating mung beans
Plant Physiol.
97
1329-1333
1991
Vigna radiata
brenda
Shinano, T.; Yonetani, R.; Ushihara, N.; Adachi, H.; Wasaki, J.; Matsui, H.; Osaki, M.
Characteristics of phosphoenolpyruvate phosphatase purified from Allium cepa
Plant Sci.
161
861-869
2001
Allium cepa
-
brenda
Singh, D.K.; Singh, R.
Partial purification and characterization of phosphoenolpyruvate phosphatase from developing seeds of Brassica campestris
Proc. Indian Acad. Sci. Sect. B
66
53-63
1996
Brassica rapa subsp. oleifera
-
brenda
Suzuki, M.; Hashioka, A.; Mimura, T.; Ashihara, H.
Salt stress and glycolytic regulation in suspension-cultured cells of the mangrove tree, Bruguiera sexangula
Physiol. Plant.
123
246-253
2005
Bruguiera sexangula
-
brenda
Obiadalla-Ali, H.; Fernie, A.R.; Kossmann, J.; Lloyd, J.R.
Developmental analysis of carbohydrate metabolism in tomato (Lycopersicon esculentum cv. Micro-Tom) fruits
Physiol. Plant.
120
196-204
2004
Solanum lycopersicum
brenda
Dong, D.; Peng, X.; Yan, X.
Organic acid exudation induced by phosphorus deficiency and/or aluminum toxicity in two contrasting soybean genotypes
Physiol. Plant.
122
190-199
2004
Glycine max
-
brenda
Smith, B.R.; Cheng, L.
Iron assimilation and carbon metabolism in Concord grapevines grown at different pHs
J. Am. Soc. Hort. Sci.
132
473-483
2007
Vitis labrusca x Vitis vinifera
-
brenda
Yang, L.; Yang, G.; You, X.; Zhou, C.; Lu, Y.; Chen, L.
Magnesium deficiency-induced changes in organic acid metabolism of Citrus sinensis roots and leaves
Biol. Plant.
57
481-486
2013
Citrus sinensis
-
brenda
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