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EC Tree
IUBMB Comments A flavoprotein. The enzyme specifically modifies uracil16 and uracil17 in tRNA.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
5,6-dihydrouracil16 in tRNA
+
=
uracil16 in tRNA
+
+
5,6-dihydrouracil17 in tRNA
+
=
uracil17 in tRNA
+
+
Synonyms
Dus1p, tRNA-dihydrouridine synthase 1,
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tRNA-dihydrouridine synthase 1
-
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5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
(1)
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
-
-
-
-
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
(2)
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
-
-
-
-
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tRNA-5,6-dihydrouracil16/17:NAD(P)+ oxidoreductase
A flavoprotein. The enzyme specifically modifies uracil16 and uracil17 in tRNA.
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uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
uracil17 in pre-tRNAPhe + NAD(P)H + H+
5,6-dihydrouracil17 in pre-tRNAPhe + NAD(P)+
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
e.g. tRNAPhe and tRNATyr
-
-
?
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uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
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FAD
stimulates activity 25fold
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-
SwissProt
brenda
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physiological function
formation of dihydrouridine in yeast cytoplasmic tRNAs is carried out by a family of four dihydrouridine synthases (Dus1p, Dus2p, Dus3p, and Dus4p), each acting at specific positions in tRNAs. Dus1p modifies U16 and U17, Dus2p modifies U20, Dus3p modifies U47, and Dus4p modifies U20a and U20b. These four proteins are responsible for all dihydrouridine modification of cytoplasmic tRNAs in yeast
malfunction
tRNAPhe from dus1-DELTA strain has little detectable dihydrouridine compared to tRNAPhe from wild-type. tRNAPhe contains dihydrouridine at position 16 and 17
malfunction
yeast extract from a dus1-DELTA strain is completely defective in modification of yeast pre-tRNAPhe
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DUS1_SCHPO
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
399
0
45327
Swiss-Prot
other Location (Reliability: 5 )
DUS1_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
423
0
48131
Swiss-Prot
other Location (Reliability: 3 )
A0A1D8N8U6_YARLL
423
0
48057
TrEMBL
other Location (Reliability: 5 )
A0A5B7AQX2_DAVIN
455
0
51533
TrEMBL
Mitochondrion (Reliability: 5 )
A0A5B7AVI8_DAVIN
448
0
50885
TrEMBL
Chloroplast (Reliability: 4 )
A0A5B7AR87_DAVIN
460
0
52167
TrEMBL
Chloroplast (Reliability: 5 )
W1QIH9_OGAPD
Ogataea parapolymorpha (strain ATCC 26012 / BCRC 20466 / JCM 22074 / NRRL Y-7560 / DL-1)
411
0
47583
TrEMBL
Mitochondrion (Reliability: 3 )
A0A5B7AUP7_DAVIN
424
0
48053
TrEMBL
other Location (Reliability: 3 )
A0A2I0AYX9_9ASPA
406
0
46337
TrEMBL
Mitochondrion (Reliability: 3 )
A0A072V047_MEDTR
105
0
11559
TrEMBL
other Location (Reliability: 5 )
A0A6J8D328_MYTCO
512
0
58596
TrEMBL
other Location (Reliability: 1 )
A0A2P6RNR1_ROSCH
426
0
48325
TrEMBL
Mitochondrion (Reliability: 5 )
A0A3G2S986_9BASI
456
0
51429
TrEMBL
other Location (Reliability: 1 )
A0A396IDU3_MEDTR
404
0
46563
TrEMBL
Secretory Pathway (Reliability: 3 )
A0A5B7AQT4_DAVIN
454
0
51460
TrEMBL
Chloroplast (Reliability: 5 )
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expression in Escherichia coli
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Xing, F.; Hiley, S.L.; Hughes, T.R.; Phizicky, E.M.
The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs
J. Biol. Chem.
279
17850-17860
2004
Saccharomyces cerevisiae (P53759), Saccharomyces cerevisiae
brenda
Xing, F.; Martzen, M.R.; Phizicky, E.M.
A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA
RNA
8
370-381
2002
Saccharomyces cerevisiae (P53759)
brenda
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1.3.1.88 tRNA-dihydrouridine16/17 synthase [NAD(P)+]
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