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Enzyme Nomenclature
Information on EC 1.3.1.88 - tRNA-dihydrouridine16/17 synthase [NAD(P)+]
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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae
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EC NUMBER 
COMMENTARY 
1.3.1.88
-
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RECOMMENDED NAME
GeneOntology No.
tRNA-dihydrouridine16/17 synthase [NAD(P)+]
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
(1)
-
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
-
-
-
-
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
(2)
-
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
tRNA-5,6-dihydrouracil16/17:NAD(P)+ oxidoreductase
A flavoprotein. The enzyme specifically modifies uracil16 and uracil17 in tRNA.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
-
formation of dihydrouridine in yeast cytoplasmic tRNAs is carried out by a family of four dihydrouridine synthases (Dus1p, Dus2p, Dus3p, and Dus4p), each acting at specific positions in tRNAs. Dus1p modifies U16 and U17, Dus2p modifies U20, Dus3p modifies U47, and Dus4p modifies U20a and U20b. These four proteins are responsible for all dihydrouridine modification of cytoplasmic tRNAs in yeast
malfunction
-
tRNAPhe from dus1-DELTA strain has little detectable dihydrouridine compared to tRNAPhe from wild-type. tRNAPhe contains dihydrouridine at position 16 and 17
malfunction
-
yeast extract from a dus1-DELTA strain is completely defective in modification of yeast pre-tRNAPhe
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
uracil17 in pre-tRNAPhe + NAD(P)H + H+
5,6-dihydrouracil17 in pre-tRNAPhe + NAD(P)+
-
-
-
-
?
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
-
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
e.g. tRNAPhe and tRNATyr
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
P53759
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
-
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
P53759
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.3.1.88)
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