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EC 1.14.14.41 Details
show Brenda entryEC number
1.14.14.41
Accepted name
(E)-2-methylbutanal oxime monooxygenase
Reaction
(1) (E)-2-methylbutanal oxime + [reduced NADPH—hemoprotein reductase] + O2 = 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH—hemoprotein reductase] + 2 H2O (overall reaction);;(1a) (E)-2-methylbutanal oxime = (Z)-2-methylbutanal oxime;;(1b) (Z)-2-methylbutanal oxime = 2-methylbutanenitrile + H2O;;(1c) 2-methylbutanenitrile + [reduced NADPH—hemoprotein reductase] + O2 = 2-hydroxy-2-methylbutanenitrile + [oxidized NADPH—hemoprotein reductase] + H2O;;(2) (E)-2-methylpropanal oxime + [reduced NADPH—hemoprotein reductase] + O2 = 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH—hemoprotein reductase] + 2 H2O (overall reaction);;(2a) (E)-2-methylpropanal oxime = (Z)-2-methylpropanal oxime;;(2b) (Z)-2-methylpropanal oxime = 2-methylpropanenitrile + H2O;;(2c) 2-methylpropanenitrile + [reduced NADPH—hemoprotein reductase] + O2 = 2-hydroxy-2-methylpropanenitrile + [oxidized NADPH—hemoprotein reductase] + H2O
Other name(s)
CYP71E7 (gene name)
Systematic name
(E)-2-methylbutanal oxime,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase
Comment
This cytochrome P-450 (heme thiolate) enzyme is involved in the biosynthesis of the cyanogenic glucosides lotaustralin and linamarin. It catalyses three different activities - isomerization of its substrate, the (E) isomer, to the (Z) isomer, dehydration, and C-hydroxylation.
History
created 2017
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.14.14.2 created 1972, deleted 1976 1.14.14.4 created 2000, deleted 2002
