EC Explorer

1.11.2.4

fatty-acid peroxygenase

fatty acid + H₂O₂ = 3- or 2-hydroxy fatty acid + H₂O

fatty acid hydroxylase (ambiguous), P450 peroxygenase, CYP152A1, P450BS, P450SPα

fatty acid:hydroperoxide oxidoreductase (RH-hydroxylating)

A cytosolic heme-thiolate protein with sequence homology to P-450 monooxygenases. Unlike the latter, it needs neither NAD(P)H, dioxygen nor specific reductases for function. Enzymes of this type are produced by bacteria (e.g. Sphingomonas paucimobilis, Bacillus subtilis). Catalytic turnover rates are high compared with those of monooxygenation reactions as well as peroxide shunt reactions catalysed by the common P-450s. A model substrate is myristate, but other saturated and unsaturated fatty acids are also hydroxylated. Oxidizes the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) and peroxygenates aromatic substrates in a fatty-acid-dependent reaction.

created 2011