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EC 1.14.19.49 Details

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EC number

1.14.19.49

Accepted name

tetracycline 7-halogenase

Reaction

tetracycline + FADH₂ + chloride + O₂ + H⁺ = 7-chlorotetracycline + FAD + 2 H₂O

Other name(s)

ctcP (gene name)

Systematic name

tetracycline:FADH₂ oxidoreductase (7-halogenating)

Comment

The enzyme, characterized from the bacterium Streptomyces aureofaciens, is a member of the flavin-dependent halogenase family. The enzyme forms a lysine chloramine intermediate on an internal lysine residue before transferring the chlorine to the substrate. It is stereo-selective for the 4S (natural) isomer of tetracycline. FADH₂ is provided by a dedicated EC 1.5.1.36, flavin reductase (NADH).

History

created 2016

EC Tree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.2 Acting on the aldehyde or oxo group of donors

1.3 Acting on the CH-CH group of donors

1.4 Acting on the CH-NH₂ group of donors

1.5 Acting on the CH-NH group of donors

1.6 Acting on NADH or NADPH

1.7 Acting on other nitrogenous compounds as donors

1.8 Acting on a sulfur group of donors

1.9 Acting on a heme group of donors

1.10 Acting on diphenols and related substances as donors

1.11 Acting on a peroxide as acceptor

1.12 Acting on hydrogen as donor

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.1 With NADH or NADPH as one donor (deleted sub-subclass)

1.14.1.1 created 1961 as EC 1.99.1.1, transferred 1965 to EC 1.14.14.1, deleted 1972

1.14.1.2 created 1965, deleted 1972

1.14.1.3 created 1961 as EC 1.99.1.13, transferred 1965 to EC 1.14.1.3, deleted 1972

1.14.1.4 created 1965, deleted 1972

1.14.1.5 created 1965, deleted 1972

1.14.1.6 created 1961 as EC 1.99.1.7, transferred 1965 to EC 1.14.1.6, deleted 1972

1.14.1.7 created 1965, deleted 1972

1.14.1.8 created 1965, deleted 1972

1.14.1.9 created 1965, deleted 1972

1.14.1.10 created 1965, deleted 1972

1.14.1.11 created 1965, deleted 1972

1.14.2 With ascorbate as one donor (deleted sub-subclass)

1.14.3 With reduced pteridine as one donor (deleted sub-subclass)

1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor

1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.16 With reduced pteridine as one donor, and incorporation of one atom of oxygen into the other donor

1.14.17 With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor

1.14.18 With another compound as one donor, and incorporation of one atom of oxygen into the other donor

1.14.19 With oxidation of a pair of donors resulting in the reduction of O₂ to two molecules of water

1.14.19.1 stearoyl-CoA 9-desaturase

1.14.19.2 stearoyl-[acyl-carrier-protein] 9-desaturase

1.14.19.3 acyl-CoA 6-desaturase

1.14.19.4 acyl-lipid (11-3)-desaturase

1.14.19.5 acyl-CoA 11-(Z)-desaturase

1.14.19.6 acyl-CoA (9+3)-desaturase

1.14.19.7 (S)-2-hydroxypropylphosphonic acid epoxidase

1.14.19.8 pentalenolactone synthase

1.14.19.9 tryptophan 7-halogenase

1.14.19.10 icosanoyl-CoA 5-desaturase

1.14.19.11 acyl-[acyl-carrier-protein] 4-desaturase

1.14.19.12 acyl-lipid ω-(9-4) desaturase

1.14.19.13 acyl-CoA 15-desaturase

1.14.19.14 linoleoyl-lipid Δ⁹ conjugase

1.14.19.15 (11Z)-hexadec-11-enoyl-CoA conjugase

1.14.19.16 linoleoyl-lipid Δ¹² conjugase (11E,13Z-forming)

1.14.19.17 sphingolipid 4-desaturase

1.14.19.18 sphingolipid 8-(E)-desaturase

1.14.19.19 sphingolipid 10-desaturase

1.14.19.20 Δ⁷-sterol 5(6)-desaturase

1.14.19.21 cholesterol 7-desaturase

1.14.19.22 acyl-lipid ω-6 desaturase (cytochrome b₅)

1.14.19.23 acyl-lipid (n+3)-(Z)-desaturase (ferredoxin)

1.14.19.24 acyl-CoA 11-(E)-desaturase

1.14.19.25 acyl-lipid ω-3 desaturase (cytochrome b₅)

1.14.19.26 acyl-[acyl-carrier-protein] 6-desaturase

1.14.19.27 sn-2 palmitoyl-lipid 9-desaturase

1.14.19.28 sn-1 stearoyl-lipid 9-desaturase

1.14.19.29 sphingolipid 8-(E/Z)-desaturase

1.14.19.30 acyl-lipid (8-3)-desaturase

1.14.19.31 acyl-lipid (7-3)-desaturase

1.14.19.32 palmitoyl-CoA 14-(E/Z)-desaturase

1.14.19.33 Δ¹² acyl-lipid conjugase (11E,13E-forming)

1.14.19.34 acyl-lipid (9+3)-(E)-desaturase

1.14.19.35 sn-2 acyl-lipid ω-3 desaturase (ferredoxin)

1.14.19.36 sn-1 acyl-lipid ω-3 desaturase (ferredoxin)

1.14.19.37 acyl-CoA 5-desaturase

1.14.19.38 acyl-lipid Δ⁶-acetylenase

1.14.19.39 acyl-lipid Δ¹²-acetylenase

1.14.19.40 hex-5-enoyl-[acyl-carrier protein] acetylenase

1.14.19.41 sterol 22-desaturase

1.14.19.42 palmitoyl-[glycerolipid] 7-desaturase

1.14.19.43 palmitoyl-[glycerolipid] 3-(E)-desaturase

1.14.19.44 acyl-CoA (8-3)-desaturase

1.14.19.45 sn-1 oleoyl-lipid 12-desaturase

1.14.19.46 sn-1 linoleoyl-lipid 6-desaturase

1.14.19.47 acyl-lipid (9-3)-desaturase

1.14.19.48 tert-amyl alcohol desaturase

1.14.19.49 tetracycline 7-halogenase

1.14.19.50 noroxomaritidine synthase

1.14.19.51 (S)-corytuberine synthase

1.14.19.52 camalexin synthase

1.14.19.53 all-trans-retinol 3,4-desaturase

1.14.19.54 1,2-dehydroreticuline synthase

1.14.19.55 4-hydroxybenzoate brominase (decarboxylating)

1.14.19.56 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] chlorinase

1.14.19.57 1H-pyrrole-2-carbonyl-[peptidyl-carrier protein] brominase

1.14.19.58 tryptophan 5-halogenase

1.14.19.59 tryptophan 6-halogenase

1.14.19.60 7-chloro-L-tryptophan 6-halogenase

1.14.19.61 dihydrorhizobitoxine desaturase

1.14.19.62 secologanin synthase

1.14.19.63 pseudobaptigenin synthase

1.14.19.64 (S)-stylopine synthase

1.14.19.65 (S)-cheilanthifoline synthase

1.14.19.66 berbamunine synthase

1.14.19.67 salutaridine synthase

1.14.19.68 (S)-canadine synthase

1.14.19.69 biflaviolin synthase

1.14.19.70 mycocyclosin synthase

1.14.19.71 fumitremorgin C synthase

1.14.19.72 (–)-pluviatolide synthase

1.14.19.73 (S)-nandinine synthase

1.14.19.74 (+)-piperitol/(+)-sesamin synthase

1.14.19.75 very-long-chain acyl-lipid ω-9 desaturase

1.14.19.76 flavone synthase II

1.14.19.77 plasmanylethanolamine desaturase

1.14.19.78 decanoyl-[acyl-carrier protein] acetylenase

1.14.19.79 3β,22α-dihydroxysteroid 3-dehydrogenase

1.14.20 With 2-oxoglutarate as one donor, and the other dehydrogenated

1.14.21 With NADH or NADPH as one donor, and the other dehydrogenated

1.14.99 Miscellaneous

1.15 Acting on superoxide as acceptor

1.15.1 Acting on superoxide as acceptor (only sub-subclass identified to date)

1.16 Oxidizing metal ions

1.17 Acting on CH or CH₂ groups

1.18 Acting on iron-sulfur proteins as donors

1.19 Acting on reduced flavodoxin as donor

1.20 Acting on phosphorus or arsenic in donors

1.21 Catalysing the reaction X-H + Y-H = X-Y

1.21.1 With NAD⁺ or NADP⁺ as acceptor

1.21.3 With oxygen as acceptor

1.21.4 With a disulfide as acceptor

1.21.98 With other, known, physiological acceptors

1.21.99 With unknown physiological acceptors

1.22 Acting on halogen in donors

1.23 Reducing C-O-C group as acceptor

1.97 Other oxidoreductases

1.98 Enzymes using H₂ as reductant (deleted subclass)

1.99 Other enzymes using O₂ as oxidant (deleted subclass)

3.1 Acting on ester bonds

3.2 Glycosylases

3.3 Acting on ether bonds

3.4 Acting on peptide bonds (peptidases)

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.6 Acting on acid anhydrides

3.7 Acting on carbon-carbon bonds

3.8 Acting on halide bonds

3.9 Acting on phosphorus-nitrogen bonds

3.10 Acting on sulfur-nitrogen bonds

3.10.1 Acting on sulfur-nitrogen bonds (only sub-subclass identified to date)

3.11 Acting on carbon-phosphorus bonds

3.12 Acting on sulfur-sulfur bonds

3.13 Acting on carbon-sulfur bonds

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Release 2023.1 (January 2023)