EC Explorer

3.1.12.2

DNA-3′-diphospho-5′-guanosine diphosphatase

[DNA]-3′-diphospho-5′-guanosine + H₂O = [DNA]-3′-phosphate + GMP

aprataxin, DNA-3′pp5′G guanylate hydrolase, APTX (gene name), HNT3 (gene name)

[DNA]-3′-diphospho-5′-guanosine hydrolase (guanosine 5′-phosphate-forming)

Aprataxin is a DNA-binding protein that catalyses (among other activities) the 3′ decapping of DNA-ppG (formed by EC 6.5.1.8, 3′-phosphate/5′-hydroxy nucleic acid ligase) [1]. The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and its eventual release. The enzyme also possesses the activity of EC 3.1.11.7, adenosine-5′-diphospho-5′-[DNA] diphosphatase, and EC 3.1.11.8, guanosine-5′-diphospho-5′-[DNA] diphosphatase.

created 2017, deleted 2019