EC Explorer

1.14.12.23

nitroarene dioxygenase

nitrobenzene + NADH + O₂ = catechol + nitrite + NAD⁺

cnbA (gene name)

nitrobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating, nitrite-releasing)

This enzyme is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin—NAD⁺ reductase), and an α3β3 oxygenase. The enzyme forms of a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It can typically act on many different nitroaromatic compounds, including chlorinated species. Enzymes found in different strains may have different substrate preferences. Requires Fe²⁺.

created 2015