EC Explorer

1.14.11.73

[protein]-arginine 3-hydroxylase

[protein]-L-arginine + 2-oxoglutarate + O₂ = [protein]-(3R)-3-hydroxy-L-arginine + succinate + CO₂

JMJD5 (gene name)

[protein]-L-arginine,2-oxoglutarate:oxygen oxidoreductase (3R-hydroxylating)

The enzyme, characterized from humans, catalyses the stereoselective formation of the (2S,3R)-hydroxy-L-arginine stereoisomer. So far the enzyme has been shown to act on two substrates - the 40S ribosomal protein S6 (RPS6), which is hydroxylated at R137, and, at a lower activity, RCCD1, a protein involved in chromatin stability, which is hydroxylated at R141. Even though the same stereoisomer is produced by the bacterial EC 1.14.11.47, [50S ribosomal protein L16]-arginine 3-hydroxylase, the two enzymes do not exhibit any cross-reactivity on their respective ribosomal protein substrates.

created 2020