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EC 1.21.3.3 Details
EC number
1.21.3.3
Accepted name
reticuline oxidase
Reaction
(S)-reticuline + O2 = (S)-scoulerine + H2O2
Other name(s)
BBE,
berberine bridge enzyme,
berberine-bridge-forming enzyme,
tetrahydroprotoberberine synthase
Systematic name
(S)-reticuline:oxygen oxidoreductase (methylene-bridge-forming)
CAS registry number
152232-28-5
Comment
Contains FAD. The enzyme from the plant Eschscholtzia californica binds the cofactor covalently [3]. Acts on (S)-reticuline and related compounds, converting the N-methyl group into the methylene bridge (’berberine bridge’) of (S)-tetrahydroprotoberberines. The product of the reaction, (S)-scoulerine, is a precursor of protopine, protoberberine and benzophenanthridine alkaloid biosynthesis in plants.
History
created 1989 as EC 1.5.3.9, transferred 2002 to EC 1.21.3.3
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