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EC 3.1.21.8 Details
EC number
3.1.21.8
Accepted name
T4 deoxyribonuclease II
Reaction
Endonucleolytic nicking and cleavage of cytosine-containing double-stranded DNA.
Other name(s)
T4 endonuclease II, EndoII (ambiguous), denA (gene name)
Comment
Requires Mg2+. This phage T4 enzyme is involved in degradation of host DNA. The enzyme primarily catalyses nicking of the bottom strand of double stranded DNA between the first and second base pair to the right of a top-strand CCGC motif. Double-stranded breaks are produced 5- to 10-fold less frequently [3]. It does not cleave the T4 native DNA, which contains 5-hydroxymethylcytosine instead of cytosine.
History
created 2014
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EC Tree
1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.2 Acting on the aldehyde or oxo group of donors
1.3 Acting on the CH-CH group of donors
1.4 Acting on the CH-NH2 group of donors
1.5 Acting on the CH-NH group of donors
1.6 Acting on NADH or NADPH
1.7 Acting on other nitrogenous compounds as donors
1.8 Acting on a sulfur group of donors
1.9 Acting on a heme group of donors
1.10 Acting on diphenols and related substances as donors
1.11 Acting on a peroxide as acceptor
1.12 Acting on hydrogen as donor
1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.15 Acting on superoxide as acceptor
1.16 Oxidizing metal ions
1.17 Acting on CH or CH2 groups
1.18 Acting on iron-sulfur proteins as donors
1.19 Acting on reduced flavodoxin as donor
1.20 Acting on phosphorus or arsenic in donors
1.21 Catalysing the reaction X-H + Y-H = X-Y
1.21.1 With NAD+ or NADP+ as acceptor
1.21.3 With oxygen as acceptor
1.21.4 With a disulfide as acceptor
1.21.98 With other, known, physiological acceptors
1.21.99 With unknown physiological acceptors
1.22 Acting on halogen in donors
1.23 Reducing C-O-C group as acceptor
1.97 Other oxidoreductases
1.98 Enzymes using H2 as reductant (deleted subclass)
1.99 Other enzymes using O2 as oxidant (deleted subclass)
2 Transferases
3 Hydrolases
3.1 Acting on ester bonds
3.1.1 Carboxylic-ester hydrolases
3.1.2 Thioester hydrolases
3.1.3 Phosphoric-monoester hydrolases
3.1.4 Phosphoric-diester hydrolases
3.1.5 Triphosphoric-monoester hydrolases
3.1.6 Sulfuric-ester hydrolases
3.1.7 Diphosphoric-monoester hydrolases
3.1.8 Phosphoric-triester hydrolases
3.1.11 Exodeoxyribonucleases producing 5′-phosphomonoesters
3.1.12 Exodeoxyribonucleases producing 3′-phosphomonoesters
3.1.13 Exoribonucleases producing 5′-phosphomonoesters
3.1.14 Exoribonucleases producing 3'-phosphomonoesters
3.1.15 Exonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters
3.1.16 Exonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters
3.1.21 Endodeoxyribonucleases producing 5'-phosphomonoesters
3.1.21.1 deoxyribonuclease I
3.1.21.2 deoxyribonuclease IV
3.1.21.3 type I site-specific deoxyribonuclease
3.1.21.4 type II site-specific deoxyribonuclease
3.1.21.5 type III site-specific deoxyribonuclease
3.1.21.6 CC-preferring endodeoxyribonuclease
3.1.21.7 deoxyribonuclease V
3.1.21.8 T4 deoxyribonuclease II
3.1.21.9 T4 deoxyribonuclease IV
3.1.21.10 crossover junction endodeoxyribonuclease
3.1.22 Endodeoxyribonucleases producing 3'-phosphomonoesters
3.1.23 Site-specific endodeoxyribonucleases: cleavage is sequence specific (deleted sub-subclass)
3.1.24 Site specific endodeoxyribonucleases: cleavage is not sequence specific (deleted sub-subclass)
3.1.25 Site-specific endodeoxyribonucleases that are specific for altered bases
3.1.26 Endoribonucleases producing 5'-phosphomonoesters
3.1.27 Endoribonucleases producing 3'-phosphomonoesters
3.1.30 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 5'-phosphomonoesters
3.1.31 Endoribonucleases that are active with either ribo- or deoxyribonucleic acids and produce 3'-phosphomonoesters
3.2 Glycosylases
3.3 Acting on ether bonds
3.4 Acting on peptide bonds (peptidases)
3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
3.6 Acting on acid anhydrides
3.7 Acting on carbon-carbon bonds
3.8 Acting on halide bonds
3.9 Acting on phosphorus-nitrogen bonds
3.10 Acting on sulfur-nitrogen bonds
3.10.1 Acting on sulfur-nitrogen bonds (only sub-subclass identified to date)
3.11 Acting on carbon-phosphorus bonds
3.12 Acting on sulfur-sulfur bonds
3.13 Acting on carbon-sulfur bonds
4 Lyases
5 Isomerases
6 Ligases
7 Translocases