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EC 3.1.11.8 Details
EC number
3.1.11.8
Accepted name
guanosine-5′-diphospho-5′-[DNA] diphosphatase
Reaction
guanosine-5′-diphospho-5′-[DNA] + H2O = phospho-5′-[DNA] + GMP
Other name(s)
aprataxin,
pp5′G5′DNA diphosphatase,
pp5′G5′-DNA guanylate hydrolase,
APTX (gene name),
HNT3 (gene name)
Systematic name
guanosine-5′-diphospho-5′-[DNA] hydrolase (guanosine 5′-phosphate-forming)
Comment
Aprataxin is a DNA-binding protein that catalyses (among other activities) the 5′ decapping of Gpp-DNA (formed by homologs of RtcB3 from the bacterium Myxococcus xanthus). The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and eventual release. The enzyme forms a 5′-phospho terminus that can be efficiently joined by "classical" ligases. The enzyme also possesses the activitiy of EC 3.1.11.7, adenosine-5′-diphospho-5′-[DNA] diphosphatase and EC 3.1.12.2, DNA-3′-diphospho-5′-guanosine diphosphatase.
History
created 2017, deleted 2019
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