3.5.3.2: guanidinoacetase

This is an abbreviated version!
For detailed information about guanidinoacetase, go to the full flat file.

Reaction

Synonyms

glycocyaminase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.3 In linear amidines

                3.5.3.2 guanidinoacetase

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Results	in table
7	Activating Compound
3	Application
1	CAS Registry Number
2	Crystallization (Commentary)
27	Inhibitors
1	Ki Value [mM]
3	KM Value [mM]
14	Metals/Ions
10	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
56	Organism
4	pH Optimum
1	pH Range
2	pH Stability
5	Purification (Commentary)
1	Reaction
1	Reaction Type
26	Reference
4	Specific Activity [micromol/min/mg]
2	Storage Stability
44	Substrates and Products (Substrate)
8	Subunits
1	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
2	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 3.5.3.2 - guanidinoacetase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-15°C, 20 mM Tris-HCl buffer, pH 8, 1.0 mM 2-mercaptoethanol, 0.1 mM MnCl2, 6months without loss of activity

Pseudomonas sp.

-

209435

4°C, 10 mM Tris-HCl buffer, pH 8, 10 mM 2-mercaptoethanol, 1.0 mM MnCl2, 70% saturated ammonium sulfate, crystalline enzyme can be stored for at least 2 months without serious loss in activity, without added Mn2+ or 2-mercaptoethanol activity rapidly lost with a half-life of about 3 days

Pseudomonas sp.

-

209435