3.5.1.62: acetylputrescine deacetylase

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For detailed information about acetylputrescine deacetylase, go to the full flat file.

Reaction

Synonyms

acetylpolyamine amidohydrolase, APAH, APAH1, APAH2, AphA, AphB, PA0321, PA1409, polyamine deacetylase

ECTree

3 Hydrolases

3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

3.5.1 In linear amides

3.5.1.62 acetylputrescine deacetylase

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Results	in table
10	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
13	General Information
7	IC50 Value
19	Inhibitors
11	KM Value [mM]
8	Metals/Ions
1	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
20	Organism
2	Pathway
26	PDB ID
4	pH Optimum
16	Protein Variants
2	Purification (Commentary)
1	Reaction
1	Reaction Type
12	Reference
1	Specific Activity [micromol/min/mg]
57	Substrates and Products (Substrate)
1	Subunits
85	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 3.5.1.62 - acetylputrescine deacetylase

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purified enzyme in complex with 5 different inhibitors, sitting drop vapor diffusion method, mixing of 500 nl of 5-10 mg/ml of protein in 20 mM Tris, pH 8.0, 50 mM NaCl, 0.01% sodium azide, 0.5-2 mM ZnCl2, and 2.5 mM inhibitor, with 500 nl of precipitant solution containing 0.2 M LiNO3, or KNO3, or LiCL, and 20% w/v PEG 3350, and equilibration against 0.1 ml of precipitant solution reservoir, 2 days, 21°C, X-ray diffraction structure determination and analysis at 1.13-1.42 A resolution

Mycoplana ramosa

Q48935

752759