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3.4.21.65: Thermomycolin

This is an abbreviated version!
For detailed information about Thermomycolin, go to the full flat file.

Word Map on EC 3.4.21.65

Reaction

Rather nonspecific hydrolysis of proteins. Preferential cleavage: Ala-/-, Tyr-/-, Phe-/- in small molecule substrates =

Synonyms

EC 3.4.21.14, EC 3.4.4.16, Proteinase, Malbranchea pulchella sulfurea extracellular, Thermomycolase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.65 Thermomycolin

General Stability

General Stability on EC 3.4.21.65 - Thermomycolin

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Autolysis, which is the dominant inactivation process below 50°C, is decreased by the presence of calcium or macromolecules
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Calcium stabilizes against autolysis
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Denaturation by freeze-drying
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Denaturation by freezing
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Extensive autolysis, especially at low calcium concentrations produces low MW peptide material
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Freezing and freeze-drying causes a considerable loss of activity
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Macromolecules and Ca2+ provide protection against significant autolysis
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No loss of activity after 12 h of agitation on a reciprocal shaker in the absence of silicone antifoam agent or in the presence of both the antifoam agent and 5% Ficoll
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Particularly thermostable in the presence of Ca2+
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Repeated freeze-drying causes insolubilization
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Urea denaturation, 8 M, is markedly inhibited by the binding of a single Ca2+
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