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3.2.1.75: glucan endo-1,6-beta-glucosidase

This is an abbreviated version!
For detailed information about glucan endo-1,6-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.75

Reaction

gentiononaose
+ 2 H2O =
gentiobiose
+
Gentiotriose
+
Gentiotetraose

Synonyms

acidic beta-1,6-glucanase, beta-(1->6)-glucanase, beta-1, 6-endoglucanase, beta-1,6-endoglucanase, beta-1,6-glucan 6-glucanohydrolase, beta-1,6-glucan hydrolase, beta-1,6-glucanase, beta-1,6-glucanase-pustulanase, beta-1-6-glucan hydrolase, BGN16.3, BT3312, endo-(1-6)-beta-D-glucanase, endo-1,6-beta-D-glucanase gene, endo-1,6-beta-glucanase, endo-beta-(1,6) glucanase, endo-beta-1,6-glucanase, gly30B, neg-1, Neg1, PUL1,6-beta-glucan, Pus30A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.75 glucan endo-1,6-beta-glucosidase

Engineering

Engineering on EC 3.2.1.75 - glucan endo-1,6-beta-glucosidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C393S
the mutant shows 0.005% activity compared to the wild type enzyme
C396S
the mutant shows 0.03% activity compared to the wild type enzyme
D286A
the mutant shows 0.27% activity compared to the wild type enzyme
H281A
the mutant shows 0.006% activity compared to the wild type enzyme
N282A
the mutant shows 0.009% activity compared to the wild type enzyme
W345A
the mutant shows 0.004% activity compared to the wild type enzyme
C393S
-
the mutant shows 0.005% activity compared to the wild type enzyme
-
D286A
-
the mutant shows 0.27% activity compared to the wild type enzyme
-
H281A
-
the mutant shows 0.006% activity compared to the wild type enzyme
-
N282A
-
the mutant shows 0.009% activity compared to the wild type enzyme
-
food industry
the enzyme can be used to hydrolyze beta-1,6-glucans of brown algae or fungal cell walls for producing gentiobiose as a high-value sugar and glucose as a fermentable sugar
additional information