3.1.1.24: 3-oxoadipate enol-lactonase

This is an abbreviated version!
For detailed information about 3-oxoadipate enol-lactonase, go to the full flat file.

Word Map on EC 3.1.1.24

Reaction

3-Oxoadipate enol-lactone
+
H2O
=
3-oxoadipate

Synonyms

carboxymethylbutenolide lactonase, beta-ketoadipate enol-lactone hydrolase, ELH I, ELH II, 3-oxoadipate enol-lactone hydrolase, CatD, 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase, PcaL, 4-methyl-3-oxoadipate enol-lactone hydrolase, mmlL, 3-oxoadipate-enol-lactonase, PcaD, enol-lactone hydrolase, ELH, RHA1_ro01338

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.24 3-oxoadipate enol-lactonase

Purification

Purification on EC 3.1.1.24 - 3-oxoadipate enol-lactonase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
168fold, 6 step purification
-
94fold, 10 step purification
-
can not be separated from 4-carboxymuconolactone decarboxylase, EC 4.1.1.44, which catalyzes the preceding reaction. The gene pcaD, which encodes the 3-oxoadipate enol-lactone hydrolase is fused to a second gene pcaC, which encodes the decarboxylase, a protocatechuate catabolic gene cluster
-
ELH I and II, 290fold, and 550fold
-
Ni2+ affinity column chromatography and Superdex75 gel filtration