3.1.1.24: 3-oxoadipate enol-lactonase

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For detailed information about 3-oxoadipate enol-lactonase, go to the full flat file.

Reaction

Synonyms

3-oxoadipate enol-lactone hydrolase, 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase, 3-oxoadipate-enol-lactonase, 4-methyl-3-oxoadipate enol-lactone hydrolase, beta-ketoadipate enol-lactone hydrolase, carboxymethylbutenolide lactonase, CatD, EC 3.1.1.16, ELH, ELH I, ELH II, enol-lactone hydrolase, mmlL, PcaD, PcaL, RHA1_ro01338

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.24 3-oxoadipate enol-lactonase

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Results	in table
5860	AA Sequence
1	CAS Registry Number
4	Cloned(Commentary)
1	Crystallization (Commentary)
9	General Information
8	Inhibitors
2	Ki Value [mM]
2	KM Value [mM]
10	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
24	Organism
10	Pathway
2	PDB ID
2	pH Optimum
4	Protein Variants
7	Purification (Commentary)
4	Reaction
1	Reaction Type
22	Reference
7	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
5	Subunits
42	Synonyms
1	Systematic Name
1	Temperature Stability [°C]

Purification

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Purification on EC 3.1.1.24 - 3-oxoadipate enol-lactonase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

168fold, 6 step purification

Pseudomonas putida

-

94279

422fold

Pseudomonas putida

-

2972, 3661

94fold, 10 step purification

Acinetobacter calcoaceticus

-

94278

can not be separated from 4-carboxymuconolactone decarboxylase, EC 4.1.1.44, which catalyzes the preceding reaction. The gene pcaD, which encodes the 3-oxoadipate enol-lactone hydrolase is fused to a second gene pcaC, which encodes the decarboxylase, a protocatechuate catabolic gene cluster

Rhodococcus opacus

-

33102

ELH I and II, 290fold, and 550fold

Acinetobacter calcoaceticus

-

94280

Ni2+ affinity column chromatography and Superdex75 gel filtration

Paraburkholderia xenovorans

Q13KT2

715929