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1.8.4.16: thioredoxin:protein disulfide reductase

This is an abbreviated version!
For detailed information about thioredoxin:protein disulfide reductase, go to the full flat file.

Word Map on EC 1.8.4.16

Reaction

a [DsbD protein] carrying a disulfide bond
+
a [protein] with reduced L-cysteine residues
=
a [DsbD protein] with reduced L-cysteine residues
 +
a [protein] carrying a disulfide bond

Synonyms

CcdA, dipZ, disulfide bond reductase, disulfide isomerase-like protein, DsbD, DsbDalpha, NmDsbD

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.4 With a disulfide as acceptor
                1.8.4.16 thioredoxin:protein disulfide reductase

Crystallization

Crystallization on EC 1.8.4.16 - thioredoxin:protein disulfide reductase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using 25% (w/v) poly(ethylene glycol) 4000, 0.1 M sodium acetate, pH 4.6, and 0.2 M NH4SO4
oxidized (ox) and reduced (red) periplasmic domains c-DsbD and n-DsbD, using 8-20% (w/v) PEG 6000, 100 mM MES (pH 6.4), and 20 mM ZnCl2 for n-DsbDox, 2.5 M ammonium sulfate and 0.1 M Tris (pH 9.1) for n-DsbDred, 5% (w/v) PEG 400 and 1.7-2.2 M citrate/citric acid (pH 7.0-7.8) for c-DsbDox, and 0.2 M zinc acetate dehydrate, 0.1 M sodium cacodylate trihydrate (pH 7.5), and 18% (w/v) PEG 8000 for c-DsbDred
-
periplasmic domains n-DsbD and c-DsbD in both redox states, hanging drop vapor diffusion method, using 18-20% (w/v) PEG 6000, 0.1 M MES pH 6.4, 0.02 M ZnCl2 (for the oxidized state of n-DsbD), 2.5 M ammonium sulfate, 0.1 M Tris pH 9.1 (for the reduced state of n-DsbD), 5% (w/v) PEG 400, 1.7-2.2 M citrate/citric acid pH 7.0-7.8 (for the oxidized state of c-DsbD), or 0.2 M zinc acetate dihydrate, 0.1 M sodium cacodylate trihydrate pH 7.5, 18% (w/v) PEG 8000 (for the reduced state of c-DsbD)
-