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1.14.13.241: 5-pyridoxate monooxygenase

This is an abbreviated version!
For detailed information about 5-pyridoxate monooxygenase, go to the full flat file.

Word Map on EC 1.14.13.241

Reaction

3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate
+
NADPH
+
H+
+
O2
=
2-(Acetamidomethylene)-3-(hydroxymethyl)succinate
+
NADP+

Synonyms

5-pyridoxate dioxygenase, 5-pyridoxate oxidase, 5-pyridoxic acid oxygenase, 5-pyridoxic-acid oxygenase, compound I oxygenase, EC 1.14.12.5

ECTree

     1 Oxidoreductases
         1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
             1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
                1.14.13.241 5-pyridoxate monooxygenase

General Stability

General Stability on EC 1.14.13.241 - 5-pyridoxate monooxygenase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity is lost on resolution with acidic ammonium sulfate and can be completely restored with FAD+, but not with FMN
-
highly sensitive to sulfhydryl reagents but not to chelating agents and is stabilized by high concentrations of mercaptoethanol and glycerol
-