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1.13.11.82: 8'-apo-carotenoid 13,14-cleaving dioxygenase

This is an abbreviated version!
For detailed information about 8'-apo-carotenoid 13,14-cleaving dioxygenase, go to the full flat file.

Reaction

8'-apo-beta-carotenal
+
O2
=
13-apo-beta-carotenone
+
2,6-dimethyldeca-2,4,6,8-tetraenedial

Synonyms

apo-carotenoid 13,14-dioxygenase, NACOX1

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.82 8'-apo-carotenoid 13,14-cleaving dioxygenase

Engineering

Engineering on EC 1.13.11.82 - 8'-apo-carotenoid 13,14-cleaving dioxygenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H170A
-
the mutant has 0.08% iron content and 2.3% activity compared to the wild type enzyme
H219A
-
the mutant has no iron content and 1.1% activity compared to the wild type enzyme
H284A
-
the mutant has 0.14% iron content and 2% activity compared to the wild type enzyme
H460A
-
the inactive mutant has no iron content
H170A
-
the mutant has 0.08% iron content and 2.3% activity compared to the wild type enzyme
-
H219A
-
the mutant has no iron content and 1.1% activity compared to the wild type enzyme
-
H284A
-
the mutant has 0.14% iron content and 2% activity compared to the wild type enzyme
-
H460A
-
the inactive mutant has no iron content
-