1.13.11.73: methylphosphonate synthase
This is an abbreviated version!
For detailed information about methylphosphonate synthase, go to the full flat file.
Word Map on EC 1.13.11.73
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1.13.11.73
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dioxygenase
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oxygenases
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nonheme
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h-abstraction
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methane
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ocean
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deprotonate
- 1.13.11.73
- dioxygenase
- oxygenases
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nonheme
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h-abstraction
- methane
-
ocean
-
deprotonate
Reaction
Synonyms
methylphosphonate synthase, mpnS
ECTree
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Engineering
Engineering on EC 1.13.11.73 - methylphosphonate synthase
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Q152D
the mutant shows weakened activity compared to the wild type enzyme
Q152H
the mutant shows weakened activity compared to the wild type enzyme
E176H
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site-directed mutagenesis, the HEPD, EC 1.13.11.72, mutant is bifunctional exhibiting the activity of both 2-hydroxyethylphosphonate dioxygenase (HEPD) and methylphosphonate synthase (MPnS). The product distribution of the mutant is sensitive to a substrate isotope effect, consistent with an isotope-sensitive branching mechanism involving a common intermediate. The introduced histidine does not coordinate the active site metal, unlike the iron-binding glutamate it replaces. More HEPD activity is observed when the reaction is carried out with (R)-2-[2-2H1]-hydroxyethylphosphonate