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1.1.1.433: sulfoacetaldehyde reductase (NADH)

This is an abbreviated version!
For detailed information about sulfoacetaldehyde reductase (NADH), go to the full flat file.

Reaction

isethionate
+
NAD+
=
2-sulfoacetaldehyde
+
NADH
+
H+

Synonyms

AH68_00250, BkTauF, BwSarD, HMPREF0179_02714, sarD, sqwF, tauF

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.433 sulfoacetaldehyde reductase (NADH)

Purification

Purification on EC 1.1.1.433 - sulfoacetaldehyde reductase (NADH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His6-MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by streptomycin sulfate, dialysis, amylose affinity chromatography, followed by MBP-tag cleavage through TEV protease, dialysis, and nickel affinity chromatography, anion exchange chromatography, and gel filtration