1.1.1.376: L-arabinose 1-dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about L-arabinose 1-dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
Synonyms
AraDH, HVO_A0330, HVO_B0032, L-arabino-aldose dehydrogenase, L-arabinose 1-dehydrogenase, L-arabinose/D-galactose 1-dehydrogenase, L-AraDH, NAD(P)-dependent L-arabinose 1-dehydrogenase
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Cofactor
Cofactor on EC 1.1.1.376 - L-arabinose 1-dehydrogenase [NAD(P)+]
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NAD+
the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+
NAD+
-
the enzyme prefers NADP+ but uses also NAD+ as a cofactor
NAD+
570fold higher affinity with NADP+ (Km: 0.0028 mM) than NAD (Km: 1.586) when L-arabinose is used as a substrate. kcat value for NADP+ is 62.3/s, which is slightly smaller than that for NAD+ (105.9/s)
NAD+
preference of NADP+ over NAD+ is significantly subjected by a pair of Ser37 and Arg38
NAD+
preference of NADP+ over NAD+, wild-type enzyme. Mutant enzyme S37D/R38A shows a complete reversal of coenzyme specificity
NAD+
the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor
NADP+
the enzyme catalyzes the oxidation of L-arabinose with both NADP+ and NAD+ as electron acceptor, with a slight preference for NADP+
NADP+
-
the enzyme prefers NADP+ but uses also NAD+ as a cofactor
NADP+
570fold higher affinity with NADP+ (Km: 0.0028 mM) than NAD (Km: 1.586) when L-arabinose is used as a substrate. kcat value for NADP+ is 62.3/s, which is slightly smaller than that for NAD+ (105.9/s)
NADP+
preference of NADP+ over NAD+ is significantly subjected by a pair of Ser37 and Arg38
NADP+
preference of NADP+ over NAD+, wild-type enzyme. Mutant enzyme S37D/R38A shows a complete reversal of coenzyme specificity
NADP+
the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor