EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
1.5.1.3 | -999 |
- |
- |
392224, 392233, 392241, 392242, 392246, 392253, 392254, 392260, 392265, 392268, 392271, 392277, 392290 |
1.5.1.3 | -999 |
- |
coupled radiometric assay |
392219 |
1.5.1.3 | -999 |
- |
ecDHFR shows a monotonous decrease of its activity with increasing pressure, down to 50% at 250 MPa |
712320 |
1.5.1.3 | -999 |
- |
enzyme assay |
392224, 392259 |
1.5.1.3 | -999 |
- |
mjDHFR loses its activity significantly even in the low-pressure region and almost completely above 100 MPa, which may be related to its low substrate-binding ability |
712320 |
1.5.1.3 | -999 |
- |
overview |
392224, 392290 |
1.5.1.3 | -999 |
- |
ppDHFR shows a monotonous decrease of its activity with increasing pressure, down to 20% at 250 MPa |
712320 |
1.5.1.3 | -999 |
- |
selenomethionine-containing mutants |
392286 |
1.5.1.3 | -999 |
- |
the activity is invariant below 75 MPa and then gradually decreases with increasing pressure down to 30% at 250 MPa |
712320 |
1.5.1.3 | -999 |
- |
the enzymatic activity of svDHFR increases with pressure to 110% at around 75 MPa and then decreases to 60% at 250 MPa |
712320 |