Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
expressed in Escherichia coli | Shewanella violacea |
expressed in Escherichia coli | Photobacterium profundum |
expressed in Escherichia coli | Moritella yayanosii |
expressed in Escherichia coli | Moritella japonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
0.0018 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
0.0019 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
0.0019 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
0.0034 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
0.0062 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
0.0198 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
0.0584 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
0.101 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
0.238 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Moritella japonica | D1MX72 | - |
- |
Moritella yayanosii | D1MX71 | - |
- |
Photobacterium profundum | D1MX70 | - |
- |
Shewanella violacea | D1MYR1 | - |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography | Escherichia coli |
using affinity chromatography | Shewanella violacea |
using affinity chromatography | Photobacterium profundum |
using affinity chromatography | Moritella yayanosii |
using affinity chromatography | Moritella japonica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ecDHFR shows a monotonous decrease of its activity with increasing pressure, down to 50% at 250 MPa | Escherichia coli |
additional information | - |
mjDHFR loses its activity significantly even in the low-pressure region and almost completely above 100 MPa, which may be related to its low substrate-binding ability | Moritella japonica |
additional information | - |
ppDHFR shows a monotonous decrease of its activity with increasing pressure, down to 20% at 250 MPa | Photobacterium profundum |
additional information | - |
the activity is invariant below 75 MPa and then gradually decreases with increasing pressure down to 30% at 250 MPa | Moritella yayanosii |
additional information | - |
the enzymatic activity of svDHFR increases with pressure to 110% at around 75 MPa and then decreases to 60% at 250 MPa | Shewanella violacea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrofolate + NADPH + H+ | - |
Escherichia coli | tetrahydrofolate + NADP+ | - |
? | |
dihydrofolate + NADPH + H+ | - |
Shewanella violacea | tetrahydrofolate + NADP+ | - |
? | |
dihydrofolate + NADPH + H+ | - |
Photobacterium profundum | tetrahydrofolate + NADP+ | - |
? | |
dihydrofolate + NADPH + H+ | - |
Moritella yayanosii | tetrahydrofolate + NADP+ | - |
? | |
dihydrofolate + NADPH + H+ | - |
Moritella japonica | tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dihydrofolate reductase | - |
Escherichia coli |
dihydrofolate reductase | - |
Shewanella violacea |
dihydrofolate reductase | - |
Photobacterium profundum |
dihydrofolate reductase | - |
Moritella yayanosii |
dihydrofolate reductase | - |
Moritella japonica |
ecDHFR | - |
Escherichia coli |
mjDHFR | - |
Moritella japonica |
myDHFR | - |
Moritella yayanosii |
ppDHFR | - |
Photobacterium profundum |
svDHFR | - |
Shewanella violacea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
25 | - |
assay at | Shewanella violacea |
25 | - |
assay at | Photobacterium profundum |
25 | - |
assay at | Moritella yayanosii |
25 | - |
assay at | Moritella japonica |
25 | - |
optimal temperature | Moritella yayanosii |
30 | - |
optimal temperature | Moritella japonica |
40 | - |
optimal temperature | Shewanella violacea |
53 | - |
optimal temperature | Photobacterium profundum |
57 | - |
optimal temperature | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.4 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
19.2 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
80.3 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
83.8 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
86.8 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
90 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
109 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
144 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
150.5 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
156 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimal pH | Moritella japonica |
7 | - |
assay at | Escherichia coli |
7 | - |
assay at | Shewanella violacea |
7 | - |
assay at | Photobacterium profundum |
7 | - |
assay at | Moritella yayanosii |
7 | - |
assay at | Moritella japonica |
7 | - |
optimal pH | Shewanella violacea |
7 | - |
optimal pH | Photobacterium profundum |
7.5 | - |
optimal pH | Moritella yayanosii |
8 | - |
optimal pH | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
600 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
800 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
1900 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
7600 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
10100 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
16700 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
25200 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
25500 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
46600 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
47400 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea |