Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
C343S | no luminescence enhancement | Agrypnus binodulus |
C343S/G344T | no luminescence enhancement | Agrypnus binodulus |
C343S/G344T/L345S | increase of luminescence activity to 100% | Agrypnus binodulus |
C343S/L345S | increase of luminescence activity | Agrypnus binodulus |
G344T | no luminescence enhancement | Agrypnus binodulus |
G344T/L345S | increase of luminescence activity to 27% | Agrypnus binodulus |
L216R | no luminescence enhancement | Agrypnus binodulus |
L345S | enhancement of luminescence | Agrypnus binodulus |
additional information | chimeric protein with firefly luciferase, EC 1.13.12.7, 4% of luminescence activity from Photinus pyralis luciferase activity | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arachidonic acid | concentration above 10 microM | Drosophila melanogaster | |
linoleic acid | concentration above 10 microM | Drosophila melanogaster | |
oleic acid | concentration above 10 microM | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00168 | - |
lauric acid | - |
Drosophila melanogaster | |
0.00188 | - |
linoleic acid | - |
Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrypnus binodulus | - |
- |
- |
Drosophila melanogaster | Q9VCC6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lauric acid | - |
Drosophila melanogaster | ? | - |
? | |
linoleic acid + ATP + CoA | - |
Drosophila melanogaster | linoleoyl-CoA + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Abll | - |
Agrypnus binodulus |
long fatty acyl-CoA synthetase | - |
Drosophila melanogaster |
luciferase-like gene | - |
Agrypnus binodulus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Drosophila melanogaster |