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Literature summary for 4.2.1.30 extracted from
- Heterologous expression and characterization of recombinant glycerol dehydratase from Klebsiella pneumoniae in Escherichia coli (2007), Biotechnol. J., 2, 736-742.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | 1,3-Propanediol is a valuable chemical intermediate,which is particularly suitable as a monomer for polycondensations to produce polyesters, polyethers and polyurethanes | Klebsiella pneumoniae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Klebsiella pneumoniae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
1,2-propanediol | - |
Klebsiella pneumoniae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol | Klebsiella pneumoniae | - |
3-hydroxypropanal + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
- |
Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
| glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alphabetagamma, 1 * 64000Da + 1 * 22000Da + 1 * 16000Da, determined by SDS-PAGE | Klebsiella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycerol dehydratase | - |
Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme B12 | - |
Klebsiella pneumoniae | |
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Release 2023.1 (January 2023)
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