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0.5
(R)-1,2-Propanediol
37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit
3.07
1,2-ethanediol
pH 8.0, 37°C
0.00002 - 7
1,2-propanediol
0.7 - 2.6
meso-2,3-butanediol
0.2 - 0.24
pentane-1,2-diol
0.25 - 0.36
Propane-1,2-diol
additional information
additional information
-
0.00002
1,2-propanediol
-
coenzyme B12
0.000021
1,2-propanediol
-
K+ as cofactor
0.09
1,2-propanediol
-
mutant I498N, pH 9.0, 42°C
0.12
1,2-propanediol
-
mutant I498M, pH 8.0, 42°C
0.14
1,2-propanediol
-
mutant Q42R, pH 9.5, 37°C
0.17
1,2-propanediol
-
mutant I498V, pH 9.0, 45°C
0.19
1,2-propanediol
-
mutant I498W, pH 8.0, 42°C. Mutant Q42D, pH 9.5, 42°C, mutant Q42E, pH 8.0, 42°C. Mutant Q42F, pH 9.5, 47°C. Mutant Q42N, pH 9.5, 42°C. Mutant Q42Y, pH 7.5, 42°C
0.2
1,2-propanediol
-
enzyme in situ, toluene-treated cells
0.24
1,2-propanediol
-
mutant I498C, pH 9.0, 40°C
0.24
1,2-propanediol
-
mutant Q42M, pH 7.5, 42°C
0.24
1,2-propanediol
-
wild-type, pH 9.0, 45°C
0.27
1,2-propanediol
-
mutant Q42K, pH 9.5, 42°C
0.31
1,2-propanediol
-
mutant Q42P, pH 9.0, 42°C
0.36
1,2-propanediol
-
enzyme in vitro
0.37
1,2-propanediol
-
mutant I498S, pH 7.5, 42°C
0.45
1,2-propanediol
-
mutant Q42S, pH 9.5, 46.7°C
0.55
1,2-propanediol
-
mutant Q42H, pH 9.0, 47°C
0.64
1,2-propanediol
-
mutant Q42I, pH 8.5, 37°C
0.65
1,2-propanediol
-
mutant Q42G, pH 7.5, 42°C
0.75
1,2-propanediol
-
mutant Q42A, pH 7.5, 42°C
0.77
1,2-propanediol
-
mutant Q42L, pH 7.5, 42°C
0.95
1,2-propanediol
-
mutant I498A, pH 9.0, 45°C
1
1,2-propanediol
-
mutant I498D, pH 8.0, 45°C
1.13
1,2-propanediol
-
mutant Q42C, pH 8.0, 42°C
1.38
1,2-propanediol
-
mutant Q42T, pH 7.5, 42°C
1.43
1,2-propanediol
pH 8.0, 37°C
1.54
1,2-propanediol
-
mutant Q42V, pH 7.5, 42°C
1.9
1,2-propanediol
-
mutant I498P, pH 8.0, 42°C
3.3
1,2-propanediol
-
mutant I498T, pH 8.5, 45°C
7
1,2-propanediol
-
in the presence of 0.5 mM coenzyme B12
8
adenosylcobalamin
-
37°C, recombinant enzyme, purification method 1 and 2
20
adenosylcobalamin
-
37°C
1.08
ethanediol
-
enzyme in situ, toluene-treated cells
1.56
ethanediol
-
enzyme in vitro
0.4
glycerol
-
mutant Q42L, pH 7.5, 42°C
0.44
glycerol
-
mutant Q42R, pH 9.5, 37°C
0.48
glycerol
pH 8.0, 37°C
0.48
glycerol
-
mutant Q42S, pH 9.5, 46.7°C
0.5
glycerol
-
mutant I498M, pH 8.0, 42°C
0.52
glycerol
-
mutant I498T, pH 8.5, 45°C
0.53
glycerol
-
enzyme in situ, toluene-treated cells
0.54
glycerol
-
enzyme in vitro
0.55
glycerol
-
mutant I498N, pH 9.0, 42°C
0.57
glycerol
-
mutant Q42W, pH 7.5, 42°C
0.58
glycerol
-
mutant I498A, pH 9.0, 45°C
0.59
glycerol
-
mutant I498V, pH 9.0, 45°C
0.59
glycerol
at pH 8.5 and 45°C
0.6
glycerol
wild type enzyme, at pH 6.0 and 37°C
0.6
glycerol
-
wild-type, pH 9.0, 45°C. Mutant I498C, pH 9.0, 40°C. Mutant Q42G, pH 7.5, 42°C. Mutant Q42N, pH 9.5, 42°C
0.6
glycerol
mutant enzyme KpG525, at pH 6.0 and 37°C
0.62
glycerol
-
mutant I498W, pH 8.0, 42°C
0.62
glycerol
-
mutant Q42E, pH 8.0, 42°C
0.66
glycerol
-
mutant Q42H, pH 9.0, 47°C
0.67
glycerol
-
mutant I498S, pH 7.5, 42°C
0.7
glycerol
-
mutant Q42P, pH 9.0, 42°C
0.7
glycerol
mutant enzyme KpG60, at pH 6.0 and 37°C
0.73
glycerol
pH 7.0, 22°C
0.82
glycerol
-
mutant Q42T, pH 7.5, 42°C
0.9
glycerol
-
mutant I498P, pH 8.0, 42°C
1.03
glycerol
-
mutant Q42Y, pH 7.5, 42°C
1.07
glycerol
-
mutant Q42I, pH 8.5, 37°C
1.1
glycerol
-
37°C, recombinant enzyme, purification method 2
1.18
glycerol
-
mutant Q42M, pH 7.5, 42°C
1.2
glycerol
-
37°C, recombinant enzyme, purification method 1
1.22
glycerol
-
mutant Q42C, pH 8.0, 42°C
1.25
glycerol
-
in the presence of 0.1 M potassium phosphate buffer
1.3
glycerol
-
mutant Q42V, pH 7.5, 42°C
1.5
glycerol
-
mutant I498D, pH 8.0, 45°C
1.8
glycerol
-
mutant Q42A, pH 7.5, 42°C
1.94
glycerol
-
mutant Q42D, pH 9.5, 42°C
2.61
glycerol
-
mutant Q42F, pH 9.5, 47°C
2.8
glycerol
-
mutant Q42K, pH 9.5, 42°C
3.3
glycerol
-
in the presence of 0.5 mM coenzyme B12
0.7
meso-2,3-butanediol
37°C, pH 7.0, mutant enzyme T200S
2.6
meso-2,3-butanediol
37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit
0.2
pentane-1,2-diol
mutant enzyme KpG525, at pH 6.0 and 37°C
0.2
pentane-1,2-diol
mutant enzyme KpG60, at pH 6.0 and 37°C
0.24
pentane-1,2-diol
wild type enzyme, at pH 6.0 and 37°C
0.25
Propane-1,2-diol
at pH 8.5 and 45°C
0.32
Propane-1,2-diol
-
37°C, recombinant enzyme, purification method 1 and 2
0.36
Propane-1,2-diol
-
37°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
inactivation kinetics of the enzyme complexes with adenosylcobalamin and its analogs
-
additional information
additional information
-
adenosylcobalamin analogs
-
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161000
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
16101
x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis
16121
-
alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid
16722
-
alpha2,beta2,gamma2, 2 * 60813 + 2 * 19549 + 2 * 16722, calculation from sequence of amino acid
17700
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
188000
-
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin
190000
-
non-denaturing PAGE
19549
-
alpha2,beta2,gamma2, 2 * 60813 + 2 * 19549 + 2 * 16722, calculation from sequence of amino acid
198000
-
gel filtration, native enzyme
21322
x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis
21355
-
2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid
21487
-
alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid
220000
-
native PAGE, 2 bands: 200000 Da and 220000 Da
229000
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
40900
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
42200
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
52000
-
4 * 52000, SDS-PAGE
60433
-
alpha2,beta2,gamma2, 2 * 60433 + 2 * 21487 + 2 * 16121, calculation from sequence of amino acid
60659
-
2! beta (alpha, gamma) 1 * 21355 (subunit A) + (1 * 60659 + 1 * 16104) (subunit B) calculation from sequence of amino acid
60700
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
60702
x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis
60813
-
alpha2,beta2,gamma2, 2 * 60813 + 2 * 19549 + 2 * 16722, calculation from sequence of amino acid
62000
2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE
83800
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
85100
-
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE
90000
-
gel filtration, in the presence of ethanolamine buffer containing 0.1 M KCl, subunit B dissociates into sub-subunits
16000
-
gamma-subunit, SDS-PAGE
16000
x * 61000 + x * 22000 + x * 16000, SDS-PAGE
16000
2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE
189000
-
gel filtration in the presence of 50 mM ethanolamine-HCl buffer, pH 8.6, subunit B
189000
-
gel filtration, in the presence of ethanolamine buffer, subunit B
200000
-
gel filtration
200000
-
native PAGE, 2 bands: 200000 Da and 220000 Da
22000
-
gel filtration, subunit A, 20 mM potassium phosphate, pH 7.7, subunit A dissociates into sub-subunits of 11000 Da
22000
-
beta-subunit, SDS-PAGE
22000
-
1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE
22000
x * 61000 + x * 22000 + x * 16000, SDS-PAGE
22000
2 * 62000 + 2 * 22000 + 2 * 16000, SDS-PAGE
61000
-
alpha-subunit, SDS-PAGE
61000
-
1 beta (alpha, gamma) 1 * 22000 (subunit A) + (1 * 61000 + 1 * 16000) (subunit B), SDS-PAGE
61000
x * 61000 + x * 22000 + x * 16000, SDS-PAGE
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Abeles, R.H.
Dehydrations requiring vitamin B12 coenzyme
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
481-497
1971
Klebsiella pneumoniae, Lactobacillus sp.
-
brenda
Poppe, L.; Retey, J.
Kinetic investigations with inhibitors that mimic the posthomolysis intermediate in the reactions of coenzyme-B12-dependent glycerol dehydratase and diol deydratase
Eur. J. Biochem.
245
398-401
1997
Citrobacter freundii
brenda
Forage, R.G.; Foster, M.A.
Glycerol fermentation in Klebsiella pneumoniae: functions of the coenzyme B12-dependent glycerol and diol dehydratases
J. Bacteriol.
149
413-419
1982
Klebsiella pneumoniae
brenda
Smiley, K.L.; Sobolov, M.
A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus
Arch. Biochem. Biophys.
97
538-543
1962
Klebsiella pneumoniae, Lactobacillus sp., Lactobacillus sp. 208-A
brenda
Schneider, Z.; Larsen, E.G.; Jacobson, G.; Johnson, B.C.; Pawelkiewicz, J.
Purification and properties of glycerol dehydrase
J. Biol. Chem.
245
3388-3396
1970
Klebsiella pneumoniae, Klebsiella pneumoniae PZH 572 Warsaw
brenda
Schneider, Z.; Johnson, B.C.
Studies on the thermal dissociation of glycerol dehydrase
Arch. Biochem. Biophys.
144
491-495
1971
Klebsiella pneumoniae, Klebsiella pneumoniae PZH 572 Warsaw
brenda
Pawelkiewicz, J.
Determination of cobamide derivates in picomole quantities by means of glycerol dehydrase from Aerobacter aerogenes
Methods Enzymol.
18C
32-34
1971
Klebsiella pneumoniae, Klebsiella pneumoniae PZH 572 Warsaw
-
brenda
Stroinski, A.; Pawelkiewicz, J.; Johnson, B.C.
Allosteric interactions in glycerol dehydratase. Purification of enzyme and effects of positive and negative cooperativity for glycerol
Arch. Biochem. Biophys.
162
321-330
1974
Klebsiella pneumoniae, Lactobacillus leichmannii, Klebsiella pneumoniae PZH 572 Warsaw
brenda
Johnson, B.C.; Stroinski, A.; Schneider, Z.
Glycerol dehydratase from Aerobacter aerogenes
Methods Enzymol.
42C
315-323
1975
Klebsiella pneumoniae, Klebsiella pneumoniae PZH 572 Warsaw
brenda
Yakusheva, M.I.; Poznanskaya, A.A.; Pospelova, T.A.; Rudakova, I.P.; Yurkevich, A.M.; Yakovlev, V.A.
Study on the mechanism of action of adenosylcobalamin-dependent glycerol dehydratase from Aerobacter aerogenes. I. Role of structural components of adenosylcobalamin the formation of the active site of glycerol dehydratase
Biochim. Biophys. Acta
484
216-235
1977
Klebsiella pneumoniae
brenda
Toraya, T.; Fukui, S.
Immunochemical evidence for the difference between coenzyme-B12-dependent diol dehydratase and glycerol dehydratase
Eur. J. Biochem.
76
285-289
1977
Citrobacter freundii, Citrobacter intermedius, Klebsiella pneumoniae, Lactobacillus sp.
brenda
Poznanskaya, A.A.; Yakusheva, M.I.; Yakolev, V.A.
Study of the mechanism of action of adenosylcobalamindependent glycerol dehydratase from Aerobacter aerogenes. II. The inactivation kinetics of glycerol dehydratase complexes with adenosylobalamin and its analogs
Biochim. Biophys. Acta
484
236-243
1977
Klebsiella pneumoniae, Klebsiella pneumoniae PZH 572 Warsaw
brenda
Forage, R.G.; Foster, M.A.
Resolution of the coenzyme B-12-dependent dehydratases of Klebsiella sp. and Citrobacter freundii
Biochim. Biophys. Acta
569
249-258
1979
Citrobacter freundii, Klebsiella pneumoniae
brenda
Toraya, T.; Kuno, S.; Fukui, S.
Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae
J. Bacteriol.
141
1439-1442
1980
Citrobacter freundii, Citrobacter intermedius, Klebsiella pneumoniae, Lactobacillus sp.
brenda
Honda, S.; Toraya, T.; Fukui, S.
In situ reactivation of glycerol-inactivated coenzyme B12-dependent enzymes, glycerol dehydratase and diol dehydratase
J. Bacteriol.
143
1458-1465
1980
Klebsiella pneumoniae, Klebsiella pneumoniae PZH 572 Warsaw
brenda
Slininger, P.J.; Bothast, R.J.; Smiley, K.L.
Production of 3-hydroxypropionaldehyde from glycerol
Appl. Environ. Microbiol.
46
62-67
1983
Lactobacillus sp.
brenda
Talarico, T.L.; Dobrogosz, W.J.
Purification and characterization of glycerol dehydratase from Lactobacillus reuteri
Appl. Environ. Microbiol.
56
1195-1197
1990
Klebsiella pneumoniae, Levilactobacillus brevis, Lentilactobacillus buchneri, Limosilactobacillus reuteri
brenda
Tobimatsu, T.; Azuma, M.; Matsubara, H.; Takatori, H.; Niida, T.; Nishimoto, K.; Satoh, H.; Hayashi, R.; Toraya, T.
Cloning, sequencing, and high level expression of the genes encoding adenosylcobalamin-dependent glycerol dehydrase of Klebsiella pneumoniae
J. Biol. Chem.
271
22352-22357
1996
Klebsiella pneumoniae
brenda
Seyfried, M; Daniel, R.; Gottschalk, G.
Cloning, sequencing, and overexpression of the genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii
J. Bacteriol.
178
5793-5796
1996
Citrobacter freundii, Klebsiella pneumoniae, Limosilactobacillus reuteri
brenda
Macis, L.; Daniel, R.; Gottschalk, G.
Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum
FEMS Microbiol. Lett.
164
21-28
1998
Citrobacter freundii, Clostridium pasteurianum
brenda
Daniel, R.; Bobik, T.A.; Gottschalk, G.
Biochemistry of coenzyme B-12-dependent glycerol and diol dehydratases and organization of the encoding genes
FEMS Microbiol. Rev.
22
553-566
1999
Citrobacter freundii, Clostridium butyricum, Clostridium pasteurianum, Klebsiella pneumoniae, Lactobacillus sp., Lentilactobacillus buchneri, Levilactobacillus brevis, Limosilactobacillus reuteri, Pantoea agglomerans
brenda
Tobimatsu, T.; Kajiura, H.; Toraya, T.
Specificities of reactivating factors for adenosylcobalamin-dependent diol dehydratase and glycerol dehydratase
Arch. Microbiol.
174
81-88
2000
Klebsiella oxytoca, Klebsiella pneumoniae
brenda
O'Brien, J.R.; Raynaud, C.; Croux, C.; Girbal, L.; Soucaille, P.; Lanzilotta, W.N.
Insight into the mechanism of the B12-independent glycerol dehydratase from Clostridium butyricum: preliminary biochemical and structural characterization
Biochemistry
43
4635-4645
2004
Clostridium butyricum
brenda
Toraya, T.
Radical catalysis of B12 enzymes: structure, mechanism, inactivation, and reactivation of diol and glycerol dehydratases
Cell. Mol. Life Sci.
57
106-127
2000
Klebsiella oxytoca, Klebsiella pneumoniae
brenda
Seifert, C.; Bowien, S.; Gottschalk, G.; Daniel, R.
Identification and expression of the genes and purification and characterization of the gene products involved in reactivation of coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii
Eur. J. Biochem.
268
2369-2378
2001
Citrobacter freundii
brenda
Yamanishi, M.; Yunoki, M.; Tobimatsu, T.; Sato, H.; Matsui, J.; Dokiya, A.; Iuchi, Y.; Oe, K.; Suto, K.; Shibata, N.; Morimoto, Y.; Yasuoka, N.; Toraya, T.
The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol
Eur. J. Biochem.
269
4484-4494
2002
Klebsiella pneumoniae
brenda
Poppe, L.; Hull, W.E.; Nitsche, R.; Graf, T.; Stupperich, E.; Retey, J.
(Hydroxyalkyl)cob(III)alamins as competitive inhibitors in coenzyme B12-dependent enzymic reactions. 1H-NMR structure analysis and kinetic studies with glycerol dehydratase and diol dehydratase
Helv. Chim. Acta
82
1250-1265
1999
Klebsiella pneumoniae
-
brenda
Tobimatsu, T.; Kajiura, H.; Yunoki, M.; Azuma, M.; Toraya, T.
Identification and expression of the genes encoding a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase
J. Bacteriol.
181
4110-4113
1999
Klebsiella pneumoniae
brenda
Kajiura, H.; Mori, K.; Tobimatsu, T.; Toraya, T.
Characterization and mechanism of action of a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase
J. Biol. Chem.
276
36514-36519
2001
Klebsiella pneumoniae
brenda
Liao, D.I.; Dotson, G.; Turner, I.Jr.; Reiss, L.; Emptage, M.
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Klebsiella pneumoniae
brenda
Liao, D.I.; Reiss, L.; Turner, I.; Dotson, G.
Structure of glycerol dehydratase reactivase: a new type of molecular chaperone
Structure
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2003
Klebsiella pneumoniae
brenda
Lin, R.; Liu, H.; Hao, J.; Cheng, K.; Liu, D.
Enhancement of 1,3-propanediol production by Klebsiella pneumoniae with fumarate addition
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Klebsiella pneumoniae, Klebsiella pneumoniae M5a1
brenda
Andres, S.; Wiezer, A.; Bendfeldt, H.; Waschkowitz, T.; Toeche-Mittler, C.; Daniel, R.
Insights into the genome of the enteric bacterium Escherichia blattae: Cobalamin (B12) biosynthesis, B12-dependent reactions, and inactivation of the gene region encoding B12-dependent glycerol dehydratase by a new Mu-Like prophage
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Shimwellia blattae
brenda
Yamada, S.; Yamada, K.; Nishikawa, N.; Hioki, R.; Nirasawa, M.; Kii, K.; Ryugo, H.; Iwama, M.; Fukuda, M.
Determination of vitamin B12 using the enzyme glycerol dehydrase
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Klebsiella pneumoniae
brenda
Wang, F.; Qu, H.; Tian, P.; Tan, T.
Heterologous expression and characterization of recombinant glycerol dehydratase from Klebsiella pneumoniae in Escherichia coli
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Klebsiella pneumoniae
brenda
Qi, X.; Sun, L.; Luo, Z.; Wu, J.; Meng, X.; Tang, Y.; Wei, Y.; Huang, R.
Rational design of glycerol dehydratase: Swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties
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Citrobacter freundii (A8CH84), Klebsiella pneumoniae (A8CIU9)
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brenda
Kajiura, H.; Mori, K.; Shibata, N.; Toraya, T.
Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases
FEBS J.
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Klebsiella oxytoca, Klebsiella pneumoniae
brenda
Lehtioe, L.; Grossmann, J.G.; Kokona, B.; Fairman, R.; Goldman, A.
Crystal structure of a glycyl radical enzyme from Archaeoglobus fulgidus
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Clostridium butyricum
brenda
Sakai, T.; Yamasaki, A.; Toyofuku, S.; Nishiki, T.; Yunoki, M.; Komoto, N.; Tobimatsu, T.; Toraya, T.
Construction and characterization of hybrid dehydratases between adenosylcobalamin-dependent diol and glycerol dehydratases
J. Nutr. Sci. Vitaminol.
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Klebsiella pneumoniae
brenda
Wang, F.; Qu, H.; Zhang, D.; Tian, P.; Tan, T.
Production of 1,3-propanediol from glycerol by recombinant E. coli using incompatible plasmids system
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Escherichia coli
brenda
Tang, X.; Tan, Y.; Zhu, H.; Zhao, K.; Shen, W.
Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli
Appl. Environ. Microbiol.
75
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2009
Clostridium butyricum (Q8GEZ8), Clostridium butyricum
brenda
Andreessen, B.; Lange, A.B.; Robenek, H.; Steinbuechel, A.
Conversion of glycerol to poly(3-hydroxypropionate) in recombinant Escherichia coli
Appl. Environ. Microbiol.
76
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Clostridium butyricum
brenda
Wang, X.D.; Zhang, X.E.; Guo, Y.C.; Zhang, Z.P.; Cao, Z.A.; Zhou, Y.F.
Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits
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31
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Klebsiella pneumoniae
brenda
Sandala, G.M.; Kovacevic, B.; Baric, D.; Smith, D.M.; Radom, L.
On the reaction of glycerol dehydratase with but-3-ene-1,2-diol
Chemistry
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2009
Citrobacter freundii
brenda
Qi, X.; Chen, Y.; Jiang, K.; Zuo, W.; Luo, Z.; Wei, Y.; Du, L.; Wei, H.; Huang, R.; Du, Q.
Saturation-mutagenesis in two positions distant from active site of a Klebsiella pneumoniae glycerol dehydratase identifies some highly active mutants
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2009
Klebsiella pneumoniae, Klebsiella pneumoniae 10018
brenda
Xu, X.; Zhang, G.; Wang, L.; Ma, B.; Li, C.
Quantitative analysis on inactivation and reactivation of recombinant glycerol dehydratase from Klebsiella pneumoniae XJPD-Li
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Klebsiella pneumoniae (Q59475 and A6NA24 and A6NA25), Klebsiella pneumoniae XJPD-Li (Q59475 and A6NA24 and A6NA25)
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brenda
Zhang, G.; Xu, X.; Li, C.; Ma, B.
Cloning, expression and reactivating characterization of glycerol dehydratase reactivation factor from Klebsiella pneumoniae XJPD-Li
World J. Microbiol. Biotechnol.
25
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2009
Klebsiella pneumoniae, Klebsiella pneumoniae XJPD-Li
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brenda
Xu, X.; Zhang, G.; Lv, B.; Yuan, Y.; Li, C.
Recombinant glycerol dehydratase from Klebsiella pneumonia XJPD-Li: induction optimization, purification and characterization
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2011
Klebsiella pneumoniae (A6NA24), Klebsiella pneumoniae (A6NA25), Klebsiella pneumoniae (Q59475), Klebsiella pneumoniae XJPD-Li (A6NA24), Klebsiella pneumoniae XJPD-Li (A6NA25), Klebsiella pneumoniae XJPD-Li (Q59475)
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brenda
Demick, J.M.; Lanzilotta, W.N.
Radical SAM activation of the B12-independent glycerol dehydratase results in formation of 5-deoxy-5-(methylthio)adenosine and not 5-deoxyadenosine
Biochemistry
50
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2011
Clostridium butyricum
brenda
Escobar, A.J.; Castano, D.M.
Classifying glycerol dehydratase by its functional residues and purifying selection in its evolution
Bioinformation
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173-176
2010
Clostridium butyricum (A9LAS8), Clostridium butyricum IBUN 158B (A9LAS8)
brenda
Qi, X.; Deng, W.; Wang, F.; Guo, Q.; Chen, H.; Wang, L.; He, X.; Huang, R.
Molecular cloning, co-expression, and characterization of glycerol dehydratase and 1,3-propanediol dehydrogenase from Citrobacter freundii
Mol. Biotechnol.
54
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2013
Citrobacter freundii (A8CH84 and A8CH91 and A8CH95), Citrobacter freundii, Citrobacter freundii AS1.1732 (A8CH84 and A8CH91 and A8CH95)
brenda
Qi, X.; Guo, Q.; Wei, Y.; Xu, H.; Huang, R.
Enhancement of pH stability and activity of glycerol dehydratase from Klebsiella pneumoniae by rational design
Biotechnol. Lett.
34
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2012
Klebsiella pneumoniae (A8CIU9), Klebsiella pneumoniae, Klebsiella pneumoniae 10018 (A8CIU9)
brenda
Liu, Y.; Gallo, A.A.; Xu, W.; Bajpai, R.; Florian, J.
CHpi interactions do not contribute to hydrogen transfer catalysis by glycerol dehydratase
J. Phys. Chem. A
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2011
Clostridium butyricum
brenda
Feliks, M.; Ullmann, G.M.
Glycerol dehydratation by the B12-independent enzyme may not involve the migration of a hydroxyl group: a computational study
J. Phys. Chem. B
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7076-7087
2012
Clostridium butyricum (Q8GEZ8)
brenda
Lim, H.G.; Noh, M.H.; Jeong, J.H.; Park, S.; Jung, G.Y.
Optimum rebalancing of the 3-hydroxypropionic acid production pathway from glycerol in Escherichia coli
ACS Synth. Biol.
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2016
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475)
brenda
Jiang, W.; Li, W.; Hong, Y.; Wang, S.; Fang, B.
Cloning, expression, mutagenesis library construction of glycerol dehydratase, and binding mode simulation of its reactivase with ligands
Appl. Biochem. Biotechnol.
178
739-752
2016
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475), Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026 (Q59476 AND O08505 AND Q59475)
brenda
Durgapal, M.; Kumar, V.; Yang, T.H.; Lee, H.J.; Seung, D.; Park, S.
Production of 1,3-propanediol from glycerol using the newly isolated Klebsiella pneumoniae J2B
Bioresour. Technol.
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2014
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475), Klebsiella pneumoniae J2B (Q59476 AND O08505 AND Q59475)
brenda
Hong, E.; Kim, J.; Ha, S.; Ryu, Y.
Improved 1,3-propanediol production by Escherichia coli from glycerol due to co-expression of glycerol dehydratase reactivation factors and succinate addition
Biotechnol. Bioprocess Eng.
20
849-855
2015
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475)
brenda
Maddock, D.J.; Gerth, M.L.; Patrick, W.M.
An engineered glycerol dehydratase with improved activity for the conversion of meso-2,3-butanediol to butanone
Biotechnol. J.
12
1700480
2017
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475), Klebsiella pneumoniae
brenda
Jiang, W.; Wang, S.; Yang, Z.; Fang, B.
B12-independent glycerol dehydratase and its reactivase from Clostridia butyricum Optimizing cloning by uniform design logic
Eng. Life Sci.
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519-524
2015
Clostridium butyricum (Q8GEZ8 AND Q8GEZ7), Clostridium butyricum VPI 1718 (Q8GEZ8 AND Q8GEZ7)
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Park, E.S.; Park, S.; Shin, J.S.
Spectrophotometric assay for sensitive detection of glycerol dehydratase activity using aldehyde dehydrogenase
J. Biosci. Bioeng.
123
528-533
2017
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475), Klebsiella pneumoniae DSM 2026 (Q59476 AND O08505 AND Q59475)
brenda
Sankaranarayanan, M.; Seol, E.; Kim, Y.; Chauhan, A.S.; Park, S.
Measurement of crude-cell-extract glycerol dehydratase activity in recombinant Escherichia coli using coupled-enzyme reactions
J. Ind. Microbiol. Biotechnol.
44
477-488
2017
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475)
brenda
Tokuyama, K.; Ohno, S.; Yoshikawa, K.; Hirasawa, T.; Tanaka, S.; Furusawa, C.; Shimizu, H.
Increased 3-hydroxypropionic acid production from glycerol, by modification of central metabolism in Escherichia coli
Microb. Cell Fact.
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64
2014
Klebsiella pneumoniae (Q59476 AND O08505 AND Q59475)
brenda
Dishisha, T.; Pereyra, L.P.; Pyo, S.H.; Britton, R.A.; Hatti-Kaul, R.
Flux analysis of the Lactobacillus reuteri propanediol-utilization pathway for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol
Microb. Cell Fact.
13
76
2014
Limosilactobacillus reuteri (A5VMB2), Limosilactobacillus reuteri DSM 20016 (A5VMB2)
brenda