Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | activation | Escherichia coli | |
GTP | less efficient than ATP | Escherichia coli | |
additional information | ATP cannot be replaced by ADP, AMP | Escherichia coli |
General Stability | Organism |
---|---|
ADP and AMP bind to and stabilize the enzyme even in the absence of Mg2+ | Escherichia coli |
ATP stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',3'-dialdehyde-ATP | i.e. adenosine 2',3'-dialdehyde triphosphate, in the presence of ATP, globin as substrate | Escherichia coli | |
adenosine 5'-(3-thiotriphosphate) | i.e. ATP-gamma-S, in the presence of ATP, globin as substrate | Escherichia coli | |
ADP | prevents activation by ATP | Escherichia coli | |
alpha,beta-methylene-ATP | in the presence of ATP, globin as substrate | Escherichia coli | |
AMP | in the presence of ATP | Escherichia coli | |
beta,gamma-methylene-ATP | in the presence of ATP, globin as substrate | Escherichia coli | |
Dio-9 | ATPase inhibitor | Escherichia coli | |
ethanol | - |
Escherichia coli | |
KCl | weak, above 0.2 M | Escherichia coli | |
additional information | termination factor rho or NaN3 | Escherichia coli | |
N,N'-dicyclohexylcarbodiimide | not | Escherichia coli | |
NaCl | weak, above 0.2 M | Escherichia coli | |
oligomycin | weak | Escherichia coli | |
phosphate | above 0.1 M | Escherichia coli | |
quercetin | - |
Escherichia coli | |
vanadate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
ATP | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can replace Mg2+-ATP | Escherichia coli | |
Ca2+ | activation, as Ca2+-ATP | Escherichia coli | |
Ca2+ | slightly less effective than Mg2+-ATP | Escherichia coli | |
diphosphate | not | Escherichia coli | |
Mg2+ | requirement | Escherichia coli | |
Mg2+ | as Mg2+-ATP | Escherichia coli | |
Mn2+ | requirement, as Mn2+-ATP | Escherichia coli | |
Mn2+ | slightly more effective than Mg2+-ATP | Escherichia coli | |
Mn2+ | can replace Mg2+-ATP | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, about 60% loss of activity within 4 days, 3 mM ATP stabilizes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | phosphate + ADP | - |
? | |
casein + H2O | alpha-casein | Escherichia coli | hydrolyzed casein | - |
? | |
casein + H2O | methylcasein | Escherichia coli | hydrolyzed casein | - |
? | |
Globin + H2O | - |
Escherichia coli | ? | - |
? | |
Methylglobin + H2O | methyl-apohemoglobin | Escherichia coli | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
1 h, 80% loss of activity, 3 mM ATP stabilizes, ADP or AMP less effectively, not Mg2+ | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement | Escherichia coli | |
ATP | activation only in the presence of Mg2+ | Escherichia coli | |
ATP | MgATP2- rather than ATP4- | Escherichia coli | |
ATP | ATP-dependent protease | Escherichia coli | |
ATP | MnATP2- or CaATP2- rather than ATP4- | Escherichia coli | |
ATP | rapid depletion of ATP prevents proteolysis | Escherichia coli | |
CTP | activation | Escherichia coli | |
CTP | less efficient than ATP | Escherichia coli | |
dATP | activation | Escherichia coli | |
dATP | supports proteolysis with 75% the efficiency of ATP | Escherichia coli | |
UTP | activation | Escherichia coli | |
UTP | less efficient than ATP | Escherichia coli |