Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae, secretion of functional enzyme to the medium from recombinant cells | Lentinula edodes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | 22% inhibition, recombinant enzyme | Lentinula edodes | |
D-glucosamine | 68% inhibition, recombinant enzyme | Lentinula edodes | |
D-glucose | 44% inhibition, recombinant enzyme | Lentinula edodes | |
D-xylose | 64% inhibition, recombinant enzyme | Lentinula edodes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
soluble starch | pH 5.3, 40°C, recombinant enzyme | Lentinula edodes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 45% activation the recombinant enzyme | Lentinula edodes | |
additional information | no effect on the recombinant enzyme by Li+, Zn2+, Mg2+, Cu+, Ca2+, and EDTA | Lentinula edodes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinula edodes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2.4% carbohydrate content, recombinant enzyme | Lentinula edodes |
proteolytic modification | cleavage of signal peptide of the recombinant enzyme expressed in Saccharomyces cerevisiae | Lentinula edodes |
Purification (Comment) | Organism |
---|---|
recombinant enzyme to homogeneity from Saccharomyces cerevisiae cell culture medium, by ammonium sulfate fractionation, and anion exchange and affinity chromatography | Lentinula edodes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | preferred substrate | Lentinula edodes | amylopectin + beta-D-glucose | - |
? | |
amylose + H2O | low activity | Lentinula edodes | amylose + beta-D-glucose | - |
? | |
soluble starch + H2O | - |
Lentinula edodes | beta-D-glucose + ? | - |
? | |
soluble starch + H2O | - |
Lentinula edodes | beta-D-glucose + soluble starch | - |
? | |
starch + H2O | 16times and 29times higher activity with soluble starch compared to wheat or corn starch granules, respectively | Lentinula edodes | starch + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLA | - |
Lentinula edodes |
glucoamylase | - |
Lentinula edodes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Lentinula edodes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to, recombinant enzyme | Lentinula edodes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4833 | - |
soluble starch | pH 5.3, 40°C, recombinant enzyme | Lentinula edodes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
recombinant enzyme | Lentinula edodes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.4 | 6.4 | stability range, recombinant enzyme | Lentinula edodes |