General Stability | Organism |
---|---|
after treatment with 4 M guanidinium chloride, gel filtration on Sephadex G-25 column results in 50% loss of activity | Thermomyces lanuginosus |
after treatment with 8 M urea, gel filtration on Sephadex G-25 column results in complete recovery of activity | Thermomyces lanuginosus |
stable to treatment with 1.5% SDS | Thermomyces lanuginosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucono-1,5-lactone | non-competitive | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermomyces lanuginosus | |
1.1 | - |
maltotriose | - |
Thermomyces lanuginosus | |
2.75 | - |
p-nitrophenyl alpha-D-glucoside | - |
Thermomyces lanuginosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
1 * 55000, SDS-PAGE | Thermomyces lanuginosus |
57000 | - |
gel filtration | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
ML-M | - |
Thermomyces lanuginosus ML-M | - |
ML-M | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 10-12% carbohydrate | Thermomyces lanuginosus |
Purification (Comment) | Organism |
---|---|
- |
Thermomyces lanuginosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucoside + H2O | - |
Thermomyces lanuginosus | 4-nitrophenol + D-glucose | - |
? | |
amylopectin + H2O | - |
Thermomyces lanuginosus | D-glucose + ? | - |
? | |
amylopectin + H2O | - |
Thermomyces lanuginosus ML-M | D-glucose + ? | - |
? | |
dextrin + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
dextrin + H2O | - |
Thermomyces lanuginosus ML-M | ? | - |
? | |
glycogen + H2O | - |
Thermomyces lanuginosus | glucose + ? | - |
? | |
glycogen + H2O | - |
Thermomyces lanuginosus ML-M | glucose + ? | - |
? | |
maltose + H2O | - |
Thermomyces lanuginosus | 2 glucose | - |
? | |
maltotriose + H2O | hydrolysis by a multi-chain mechanism | Thermomyces lanuginosus | maltose + glucose | - |
? | |
additional information | no hydrolysis of alpha-1,6 linkages | Thermomyces lanuginosus | ? | - |
? | |
additional information | no hydrolysis of alpha-1,6 linkages | Thermomyces lanuginosus ML-M | ? | - |
? | |
starch + H2O | soluble | Thermomyces lanuginosus | glucose + ? | - |
? | |
starch + H2O | soluble | Thermomyces lanuginosus ML-M | glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
6 h, completly stable | Thermomyces lanuginosus |
60 | - |
pH 6.0, 6 h, about 40% loss of activity, half-life: 7.3 h | Thermomyces lanuginosus |
70 | - |
pH 6.0, half-life: 30 min | Thermomyces lanuginosus |
80 | - |
pH 6.0, half-life: 10 min | Thermomyces lanuginosus |