Crystallization (Comment) | Organism |
---|---|
by the hanging-drop method, 6PGL in complex with two ligands, 6-phosphogluconic acid and citrate, at 2.2 A and 2.0 A resolution, respectively. Structures of the two complexes and of the apo form are almost identical. Both complexes belong to space group P21 and have two molecules in the asymmetric unit, related by a pure translation. PGL in complex with 6-phosphogluconic acid has unit cell parameters of a = 49.66, b = 87, c= 64.91 A and beta = 96.43. PGL in complex with citrate has unit cell parameters of a = 49.46, b = 87.21, c= 64.79 A and beta = 96.53 | Trypanosoma brucei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | has a structural role rather than a catalytic one, lies at the center of a tetrahedron. The side chains of H56 and H97 are at a distance of 2.0 A from the metal ion, while the oxygen atom of the carboxylic side chain of D98 coordinates the zinc ion in a monodentate manner. Water molecule at 2.7 A from the metal ion | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to 95% homogeneity in a single step with an NTA column | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-glucono-1,5-lactone + H2O | H165 is the catalytic residue, while R77 and R200 bind to the phosphate group. Ring opening of the lactone involves proton transfer from HE2 of H165 to O1 or O5 of the lactone, via a water molecule | Trypanosoma brucei | 6-phospho-D-gluconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphogluconolactonase | - |
Trypanosoma brucei |
6PGL | - |
Trypanosoma brucei |