Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.5-6%, absolute requirement | Escherichia coli |
General Stability | Organism |
---|---|
combination of both 4 M urea and 1% SDS at 30°C completely inactivates | Escherichia coli |
SDS, 1%, 50% loss of activity after 2 h at 30°C | Escherichia coli |
urea, 8 M, stable in presence of 0.1% Triton X-100 after 2 h at 30°C | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP-diacylglycerol | inhibition of CDP-diacylglycerol formation | Escherichia coli | |
glycerol 3-phosphate | inhibition of CDP-diacylglycerol formation | Escherichia coli | |
Liponucleotide | forms a dead-end complex at high concentrations inhibiting both, the forward and the reverse reaction | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
dCDP-diacylglycerol | 30°C, pH 8.0 | Escherichia coli | |
0.046 | - |
CDP-diacylglycerol | 30°C, pH 8.0 | Escherichia coli | |
0.32 | - |
sn-glycerol 3-phosphate | 30°C, pH 8.0 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated | Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
x * 24000, SDS-PAGE | Escherichia coli |
200000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 1-(3-sn-phosphatidyl)-sn-glycerol 3-phosphate | ordered sequential bi-bi reaction | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + sn-glycero-3-phosphate | - |
Escherichia coli | CMP + phosphatidylglycerophosphate | 3-sn-phosphatidyl-1'-sn-glycerol 3'-phosphate | r | |
dCDP-diacylglycerol + sn-glycero-3-phosphate | - |
Escherichia coli | dCMP + phosphatidylglycerophosphate | - |
? | |
additional information | cytidine 5'-monophosphate dependent exchange between glycerol 3-phosphate and phosphatidylglycerophosphate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, stable, partially purified enzyme, Triton X-100 extract | Escherichia coli |
60 | - |
5 min, 50% loss of activity, partially purified enzyme, Triton X-100 extract | Escherichia coli |
65 | - |
5 min, complete loss of activity, partially purified enzyme, Triton X-100 extract | Escherichia coli |