Information on EC 2.7.8.5 - CDP-diacylglycerol-glycerol-3-phosphate 1-phosphatidyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.8.5
-
RECOMMENDED NAME
GeneOntology No.
CDP-diacylglycerol-glycerol-3-phosphate 1-phosphatidyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 1-(3-sn-phosphatidyl)-sn-glycerol 3-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
substituted phospho group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cardiolipin biosynthesis I
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cardiolipin biosynthesis II
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cardiolipin biosynthesis III
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Glycerophospholipid metabolism
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Metabolic pathways
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phosphatidylglycerol biosynthesis I (plastidic)
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phosphatidylglycerol biosynthesis II (non-plastidic)
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type I lipoteichoic acid biosynthesis (S. aureus)
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cardiolipin biosynthesis
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lipid metabolism
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SYSTEMATIC NAME
IUBMB Comments
CDP-diacylglycerol:sn-glycerol-3-phosphate 1-(3-sn-phosphatidyl)transferase
The enzyme catalyses the committed step in the biosynthesis of acidic phospholipids known by the common names phophatidylglycerols and cardiolipins.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-49-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain WB
UniProt
Manually annotated by BRENDA team
strain WB
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-1,2-diacyl-sn-glycerol + glycerol 3-phosphate
?
show the reaction diagram
CDP-1,2-diacyl-sn-glycerol + glycerol 3-phosphate
CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the first committed step in synthesis of cardiolipid. In response to inositol, decrease in Pgs1p enzyme activity is associated with increased phosphorylation of Pgs1p. Phosphorylation of a phospholipid biosynthetic enzyme in response to inositol, new mechanism of inositol-mediated regulation
-
-
?
CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate
phosphatidylglycerophosphate + CMP
show the reaction diagram
CDP-diacylglycerol + sn-glycero-3-phosphate
CMP + phosphatidylglycerophosphate
show the reaction diagram
CDP-diacylglycerol + sn-glycerol 3-phosphate
CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate
show the reaction diagram
dCDP-diacylglycerol + sn-glycero-3-phosphate
dCMP + phosphatidylglycerophosphate
show the reaction diagram
-
-
-
-
?
DL-2-hexadecoxy-3-octadecoxypropylphosphonyl-O-(cytidine 5'-phosphate) + sn-glycero-3-phosphate
? + phosphatidylglycerophosphate
show the reaction diagram
-
-
-
-
?
DL-3,4-dioctadecoxybutylphosphonyl-O-(cytidine 5'-phosphate) + sn-glycerol 3-phosphate
? + phosphatidylglycerophosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CDP-1,2-diacyl-sn-glycerol + glycerol 3-phosphate
?
show the reaction diagram
CDP-1,2-diacyl-sn-glycerol + glycerol 3-phosphate
CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate
show the reaction diagram
-
the enzyme catalyzes the first committed step in synthesis of cardiolipid. In response to inositol, decrease in Pgs1p enzyme activity is associated with increased phosphorylation of Pgs1p. Phosphorylation of a phospholipid biosynthetic enzyme in response to inositol, new mechanism of inositol-mediated regulation
-
-
?
CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate
phosphatidylglycerophosphate + CMP
show the reaction diagram
Q9Z2Z7
cardiolipin (phospholipid) biosynthesis, regulation by short chain cell-permeable ceramide (C2-Cer: N-acetylsphingosine) signalling and levels of active GTP-bound RhoA
-
-
?
CDP-diacylglycerol + sn-glycerol 3-phosphate
CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
cation requirement is relatively nonspecific, Mg2+, Ba2+ or Ca2+ provides maximal activation in the 10 mM range, Mn2+ or Co2+ stimulates at lower concentration, inhibition at higher concentration
Cd2+
-
inhibitory
Zn2+
-
inhibitory
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CDP-diacylglycerol
Cu2+
-
strong inhibition at concentrations above 0.5 mM in presence of Mg2+
glycerol 3-phosphate
-
inhibition of CDP-diacylglycerol formation
Hg2+
-
strong inhibition at concentrations above 0.5 mM in presence of Mg2+
Inositol
-
inhibits CMP-dependent incorporation of glycerol 3-phosphate by microsomes
Liponucleotide
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forms a dead-end complex at high concentrations inhibiting both, the forward and the reverse reaction
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Thioreactive agents
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slight inhibition
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Triton X-100
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-(4-chlorophenylthio)-cAMP
-
i.e. CPT-cAMP, stimulation
Ca2+
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at 6 mM, 2.3fold stimulation
N-acetylsphingosine
-
stimulation
phosphatidylethanolamine
-
stimulates, no stimulation by other diacylglycerophosphatides or lysophosphatides
phosphatidylinositol
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required for CMP-dependent incorporation of glycerol 3-phosphate
thyroxine
-
treated of animals for 5 consecutive days with thyroxine at 250 mg per kg of body weight results in enzyme stimulation up to 3.5fold
Triton X-100
Tumor necrosis factor alpha
-
stimulation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.04
(d)CDP-diacylglycerol
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37C, pH 8.0
0.012 - 0.288
CDP-diacylglycerol
0.034
dCDP-diacylglycerol
0.08
DL-2-Hexadecoxy-3-octadecoxypropylphosphonyl-O-(cytidine 5'-phosphate)
-
37C
0.06
DL-3,4-Dioctadecoxybutylphosphonyl-O-(cytidine 5'-phosphate)
-
37C
0.02 - 0.17
glycerol 3-phosphate
0.1 - 0.32
sn-glycerol 3-phosphate
additional information
additional information
-
anomalous kinetics for CDP-diacylglycerol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000191
parental CHO-K1 cell line, mitochondrial fraction
0.000192
CHO-K1 cell line E91, mitochondrial fraction
0.000197
CHO-K1 cell line E91, N-acetylsphingosine-treated, no change in activity compared to untreated cells, mitochondrial fraction
0.000299
or 76% increased activity in parental CHO-K1 cell line, N-acetylsphingosine-treated, mitochondrial fraction
0.00127
30% decrease in activity in cells grown in presence of myo-inositol (1 h, 75 microM), mitochondrial extracts
0.0018
mitochondrial extracts
0.03
-
30C, pH 8.0
0.36
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pH 7.0, 30C
1.17
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30C, pH 7.5
12
-
37C, pH 7.4
18.6
-
37C, pH 8.0
22
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37C, pH 8.0
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5
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2 optima: pH 7.0 and 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
chinese hamster ovary parental cell line (2.5fold induction of PGP synthase mRNA in response to N-acetylsphingosine-treatment, qRT-PCR) and etoposide-/N-acetylsphingosine-resistant cell line E91 (defect in ceramide signalling and RhoGAP activity, non-significant 1.2fold induction of PGP synthase mRNA in response to N-acetylsphingosine-treatment, qRT-PCR)
Manually annotated by BRENDA team
similar mRNA levels in cells progressing from exponential to stationary phase when grown on glucose, glycerol and/or ethanol as C source and in presence or absence of phospholipid precursors myo-inositol and/or choline
Manually annotated by BRENDA team
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myoblastic cell line
Manually annotated by BRENDA team
highest expression level of PGS1 mRNA
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
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x * 24000, SDS-PAGE
120000
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gel filtration
200000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 60000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
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in response to inositol, decrease in Pgs1p enzyme activity is associated with increased phosphorylation of Pgs1p. Phosphorylation of a phospholipid biosynthetic enzyme in response to inositol, new mechanism of inositol-mediated regulation
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
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5 min, complete loss of activity, partially purified enzyme, Triton X-100 extract
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
combination of both 4 M urea and 1% SDS at 30C completely inactivates
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SDS, 1%, 50% loss of activity after 2 h at 30C
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urea, 8 M, stable in presence of 0.1% Triton X-100 after 2 h at 30C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, 50 mM Tris-HCl, pH 7.0, 10 mM MgCl2, 2 mM DTT, 0.1% Triton X-100, stable for at least 3 years
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4C, 50 mM Tris-HCl, pH 7.0, 10 mM MgCl2, 2 mM DTT, 0.1% Triton X-100, stable for several months
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4C, 50% loss of activity after 12 h, broken cell preparation
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
BB0721 gene is transformed into Pgs- mutant of Escherichia coli containing a copy of the native gene on a temperature-regulated plasmid
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gene PGS1, quantitative PCR enzyme expression analysis, recombinant overexpression of FLAG-tagged PGS1 in HEK-293 cells
gene pgsA and pgsA2, amino acid sequence comparisons, quantitative RT-PCR expression analysis
phosphatidylglycerophosphate synthase is encoded by PGP1 and PGP2 in Arabidopsis thaliana
the CgPGS1 gene is cloned into a centromeric pFL38 vector
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the integration plasmid pHT01 is constructed by insertion of the PCR amplified fragment of pgsA, encoding phosphatidylglycerophosphate synthase, into pMUTINCC
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the ScPGS1 gene is cloned in the pCgACU5 plasmid
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
partial functional complementation of Synechocystis sp. PCC6803 gene pgsA mutant individually by CrPGP1 and CrPGP2 (pgsA/CrPGP1 and pgsA/CrPGP2) rescueing the phosphatidylglycerophosphate-dependent growth phenotype, but the phosphatidylglycerophosphate level and its fatty acid composition are not fully rescued in the complemented strains. As well, oxygen evolution activity is not fully recovered, although electron transport activity of photosystem II is restored to the wild-type level, differential response of CrPGP1 and CrPGP2 expression to phosphorus and nitrogen deficiency, overview. Cloning in vector which is designed to incorporate a gene of interest at a neutral site (slr2031) with the psbA2 (slr1311) promoter and a chloramphenicol-resistance cassette
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
treatment of animals for 5 consecutive days with thyroxine at 250 mg per kg of body weight results in enzyme stimulation up to 3.5fold
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