General Stability | Organism |
---|---|
ultrafiltration or ammonium sulfate precipitation results in heavy loss of activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-mannose 1-phosphate | 1.25 mM: 22% inhibition, 4.2 mM: 40% inhibition | Saccharomyces cerevisiae | |
Mg2+ | 17 mM: 32% inhibition | Saccharomyces cerevisiae | |
phosphate | 4.5 mM: 55% inhibition | Saccharomyces cerevisiae | |
Zn2+ | 5 mM: complete inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
phosphate | pH 8.0, 30°C | Saccharomyces cerevisiae | |
0.25 | - |
UDP-mannose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
1.5 | - |
UDP-glucose | pH 8.0, 30°C | Saccharomyces cerevisiae | |
6.2 | - |
GDP-mannose | pH 8.0, 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
protoplast | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | 0.1-1 mM of Mg2+ or 1 mM of Zn2+ has no effect | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP + aldose 1-phosphate | Saccharomyces cerevisiae | - |
NDP-aldose + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.178 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, concentrated enzyme fraction, stable for several months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-mannose + phosphate | 30% of activity compared to GDPmannose | Saccharomyces cerevisiae | ADP + D-mannose 1-phosphate | - |
ir | |
dTDP-glucose + phosphate | 11% of activity compared to GDPmannose | Saccharomyces cerevisiae | dTDP + D-glucose 1-phosphate | - |
ir | |
dTDP-mannose + phosphate | 157% of activity compared to GDPmannose | Saccharomyces cerevisiae | dTDP + D-mannose 1-phosphate | - |
ir | |
GDP-galactose + phosphate | 3% of activity compared to GDPmannose | Saccharomyces cerevisiae | GDP + D-galactose 1-phosphate | - |
ir | |
GDP-mannose + phosphate | - |
Saccharomyces cerevisiae | GDP + D-mannose 1-phosphate | - |
ir | |
additional information | no activity with GDP-glucose, ADP-glucose, ADP-galactose, ADP-xylose, dADP-glucose, IDP-glucose | Saccharomyces cerevisiae | ? | - |
? | |
NDP + aldose 1-phosphate | - |
Saccharomyces cerevisiae | NDP-aldose + phosphate | - |
ir | |
UDP-acetylglucosamine + phosphate | 5.1% of activity compared to GDPmannose | Saccharomyces cerevisiae | UDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
ir | |
UDP-galactose + phosphate | 18% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-galactose 1-phosphate | - |
ir | |
UDP-glucose + phosphate | 22% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-glucose 1-phosphate | - |
ir | |
UDP-mannose + phosphate | 290% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-mannose 1-phosphate | - |
ir | |
UDP-xylose + phosphate | 24.5% of activity compared to GDP-mannose | Saccharomyces cerevisiae | UDP + D-xylose 1-phosphate | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
15 min: 40% remaining activity, 30 min: 27% remaining activity | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | 14% of maximal activity at pH 6.5, 43% of maximal activity at pH 9.5 | Saccharomyces cerevisiae |