Information on EC 2.7.7.37 - aldose-1-phosphate nucleotidyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
2.7.7.37
-
RECOMMENDED NAME
GeneOntology No.
aldose-1-phosphate nucleotidyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NDP + alpha-D-aldose 1-phosphate = phosphate + NDP-aldose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
UDP-beta-L-arabinose biosynthesis II (from beta-L-arabinose)
-
-
SYSTEMATIC NAME
IUBMB Comments
NDP:alpha-D-aldose-1-phosphate nucleotidyltransferase
The enzyme works on a variety of alpha-D-aldose 1-phosphates and beta-L-aldose 1-phosphates (which have the same anomeric configuration as the former; see 2-Carb-6.2).
CAS REGISTRY NUMBER
COMMENTARY hide
9033-61-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-mannose + phosphate
ADP + D-mannose 1-phosphate
show the reaction diagram
-
30% of activity compared to GDPmannose
-
-
ir
dTDP-glucose + phosphate
dTDP + D-glucose 1-phosphate
show the reaction diagram
-
11% of activity compared to GDPmannose
-
-
ir
dTDP-mannose + phosphate
dTDP + D-mannose 1-phosphate
show the reaction diagram
-
157% of activity compared to GDPmannose
-
-
ir
GDP-galactose + phosphate
GDP + D-galactose 1-phosphate
show the reaction diagram
-
3% of activity compared to GDPmannose
-
-
ir
GDP-mannose + phosphate
GDP + D-mannose 1-phosphate
show the reaction diagram
-
-
-
ir
NDP + aldose 1-phosphate
NDP-aldose + phosphate
show the reaction diagram
-
-
-
ir
UDP-acetylglucosamine + phosphate
UDP + N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
-
5.1% of activity compared to GDPmannose
-
-
ir
UDP-galactose + phosphate
UDP + D-galactose 1-phosphate
show the reaction diagram
-
18% of activity compared to GDP-mannose
-
-
ir
UDP-glucose + phosphate
UDP + D-glucose 1-phosphate
show the reaction diagram
-
22% of activity compared to GDP-mannose
-
-
ir
UDP-mannose + phosphate
UDP + D-mannose 1-phosphate
show the reaction diagram
-
290% of activity compared to GDP-mannose
-
-
ir
UDP-xylose + phosphate
UDP + D-xylose 1-phosphate
show the reaction diagram
-
24.5% of activity compared to GDP-mannose
-
-
ir
additional information
?
-
-
no activity with GDP-glucose, ADP-glucose, ADP-galactose, ADP-xylose, dADP-glucose, IDP-glucose
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NDP + aldose 1-phosphate
NDP-aldose + phosphate
show the reaction diagram
-
-
-
ir
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
0.1-1 mM of Mg2+ or 1 mM of Zn2+ has no effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-mannose 1-phosphate
-
1.25 mM: 22% inhibition, 4.2 mM: 40% inhibition
Mg2+
-
17 mM: 32% inhibition
phosphate
-
4.5 mM: 55% inhibition
Zn2+
-
5 mM: complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.2
GDP-mannose
-
pH 8.0, 30°C
0.12
phosphate
-
pH 8.0, 30°C
1.5
UDP-glucose
-
pH 8.0, 30°C
0.25
UDP-mannose
-
pH 8.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.5
-
14% of maximal activity at pH 6.5, 43% of maximal activity at pH 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
15 min: 40% remaining activity, 30 min: 27% remaining activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ultrafiltration or ammonium sulfate precipitation results in heavy loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, concentrated enzyme fraction, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE