Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloroethylthiamine | weak | Paracoccus denitrificans | |
iodoacetamide | 3.3 mM, 23.3% inhibition, activity is recovered to approximately 90% on incubation with a 10fold excess of dithiothreitol | Paracoccus denitrificans | |
oxythiamine | weak | Paracoccus denitrificans | |
PCMB | 1 mM, 55.1% inhibition, activity is recovered to approximately 90% on incubation with a 10fold excess of dithiothreitol | Paracoccus denitrificans | |
pyrithiamine | - |
Paracoccus denitrificans | |
thiamine diphosphate | - |
Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
thiamine | pH 9.0, 37°C | Paracoccus denitrificans | |
0.38 | - |
ATP | pH 9.0, 37°C | Paracoccus denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Paracoccus denitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Paracoccus denitrificans |
96000 | - |
aggregate of the functional dimer, equilibrium sedimentation | Paracoccus denitrificans |
230000 | - |
2 * 230000, SDS-PAGE | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Paracoccus denitrificans 12442 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paracoccus denitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0235 | - |
- |
Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + thiamine | - |
Paracoccus denitrificans | AMP + thiamine diphosphate | - |
? | |
ATP + thiamine | - |
Paracoccus denitrificans 12442 | AMP + thiamine diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 230000, SDS-PAGE | Paracoccus denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Paracoccus denitrificans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
pyrithiamine | - |
Paracoccus denitrificans |