Crystallization (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
- |
Streptomyces wedmorensis |
Protein Variants | Comment | Organism |
---|---|---|
K204A | kinetic values similar to wild-type | Thermoplasma acidophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | competitive versus isopentenyl phosphate | Streptomyces wedmorensis | |
fosfomycin | competitive versus isopentenyl phosphate | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
isopentenyl phosphate | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum | |
0.0044 | - |
isopentenyl phosphate | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum | |
0.006 | - |
ATP | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum | |
0.0069 | - |
ATP | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum | |
0.11 | - |
fosfomycin | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum | |
0.15 | - |
fosfomycin | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + isopentenyl phosphate | Thermoplasma acidophilum | - |
ADP + isopentenyl diphosphate | - |
? | |
ATP + isopentenyl phosphate | Streptomyces wedmorensis | - |
ADP + isopentenyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces wedmorensis | Q56187 | - |
- |
Thermoplasma acidophilum | Q9HLX1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + fosfomycin | i.e. [(2R,3S)-3-methyl-2-oxiranyl]phosphonic acid | Thermoplasma acidophilum | ADP + ((2R,3S)-3-methyloxiran-2-yl)phosphonic phosphoric anhydride | - |
? | |
ATP + fosfomycin | i.e. [(2R,3S)-3-methyl-2-oxiranyl]phosphonic acid | Streptomyces wedmorensis | ADP + ((2R,3S)-3-methyloxiran-2-yl)phosphonic phosphoric anhydride | - |
? | |
ATP + isopentenyl phosphate | - |
Thermoplasma acidophilum | ADP + isopentenyl diphosphate | - |
? | |
ATP + isopentenyl phosphate | - |
Thermoplasma acidophilum | ADP + isopentenyl diphosphate | - |
r | |
ATP + isopentenyl phosphate | - |
Streptomyces wedmorensis | ADP + isopentenyl diphosphate | - |
? | |
ATP + isopentenyl phosphate | - |
Streptomyces wedmorensis | ADP + isopentenyl diphosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
fosfomycin | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum | |
0.04 | - |
fosfomycin | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum | |
7.7 | - |
isopentenyl phosphate | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum | |
8 | - |
isopentenyl phosphate | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
fosfomycin | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum | |
0.0026 | - |
fosfomycin | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum | |
1800 | - |
isopentenyl phosphate | pH 7.5, 37°C, wild-type | Thermoplasma acidophilum | |
2800 | - |
isopentenyl phosphate | pH 7.5, 37°C, mutant K204A | Thermoplasma acidophilum |